CPLM-006390

Genbank Nucleotide ID

Macrophage migration inhibitory factor

Protein Synonyms/Alias

MIF; Delayed early response protein 6; DER6; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

Mus musculus (Mouse)

Position	Peptide	Type	References
78	AQNRNYSKLLCGLLS	acetylation	[1, 2, 3, 4, 5, 6]
78	AQNRNYSKLLCGLLS	succinylation	[6]
78	AQNRNYSKLLCGLLS	ubiquitination	[7]

[1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[7] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).

ACT_SITE 2 2 Proton acceptor; via imino nitrogen.
BINDING 33 33 Substrate.
BINDING 65 65 Substrate; via amide nitrogen (By
BINDING 98 98 Substrate.
MOD_RES 78 78 N6-acetyllysine (By similarity).

3D-structure; Acetylation; Complete proteome; Cytokine; Cytoplasm; Direct protein sequencing; Immunity; Inflammatory response; Innate immunity; Isomerase; Reference proteome; Secreted.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0009986; C:cell surface; IEA:Compara.
GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005615; C:extracellular space; IDA:BHF-UCL.
GO:0042056; F:chemoattractant activity; IEA:Compara.
GO:0005125; F:cytokine activity; IDA:BHF-UCL.
GO:0004167; F:dopachrome isomerase activity; ISS:UniProtKB.
GO:0050178; F:phenylpyruvate tautomerase activity; IMP:MGI.
GO:0007569; P:cell aging; IMP:MGI.
GO:0008283; P:cell proliferation; IEA:Compara.
GO:0007166; P:cell surface receptor signaling pathway; IEA:Compara.
GO:0030330; P:DNA damage response, signal transduction by p53 class mediator; IMP:MGI.
GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
GO:0090344; P:negative regulation of cell aging; IEA:Compara.
GO:0071157; P:negative regulation of cell cycle arrest; IEA:Compara.
GO:0032269; P:negative regulation of cellular protein metabolic process; IDA:BHF-UCL.
GO:0043518; P:negative regulation of DNA damage response, signal transduction by p53 class mediator; IDA:BHF-UCL.
GO:0010629; P:negative regulation of gene expression; IEA:Compara.
GO:0002906; P:negative regulation of mature B cell apoptotic process; IDA:BHF-UCL.
GO:0033033; P:negative regulation of myeloid cell apoptotic process; IMP:BHF-UCL.
GO:0002821; P:positive regulation of adaptive immune response; NAS:BHF-UCL.
GO:0090238; P:positive regulation of arachidonic acid secretion; IDA:BHF-UCL.
GO:0030890; P:positive regulation of B cell proliferation; IEA:Compara.
GO:2000343; P:positive regulation of chemokine (C-X-C motif) ligand 2 production; IDA:BHF-UCL.
GO:0050715; P:positive regulation of cytokine secretion; IEA:Compara.
GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:BHF-UCL.
GO:0048146; P:positive regulation of fibroblast proliferation; IDA:BHF-UCL.
GO:0031666; P:positive regulation of lipopolysaccharide-mediated signaling pathway; IMP:BHF-UCL.
GO:0043406; P:positive regulation of MAP kinase activity; IDA:BHF-UCL.
GO:0061081; P:positive regulation of myeloid leukocyte cytokine production involved in immune response; IMP:BHF-UCL.
GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IDA:BHF-UCL.
GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:BHF-UCL.
GO:0061078; P:positive regulation of prostaglandin secretion involved in immune response; IMP:BHF-UCL.
GO:0010739; P:positive regulation of protein kinase A signaling cascade; IEA:Compara.
GO:0001516; P:prostaglandin biosynthetic process; IEA:Compara.
GO:0070207; P:protein homotrimerization; IEA:Compara.

IPR001398; Macrophage_inhib_fac.
IPR019829; Macrophage_inhib_fac_CS.
IPR014347; Tautomerase/MIF_sf.