CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015404
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chondroitin sulfate proteoglycan 4 
Protein Synonyms/Alias
 Chondroitin sulfate proteoglycan NG2; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan 
Gene Name
 CSPG4 
Gene Synonyms/Alias
 MCSP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
2263DVQVLTAKPRNGLAGubiquitination[1, 2]
2316RTPNPALKNGQYWV*ubiquitination[1, 3]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. 
Sequence Annotation
 DOMAIN 30 192 Laminin G-like 1.
 DOMAIN 202 380 Laminin G-like 2.
 REPEAT 430 523 CSPG 1.
 REPEAT 556 643 CSPG 2.
 REPEAT 665 764 CSPG 3.
 REPEAT 792 878 CSPG 4.
 REPEAT 900 989 CSPG 5.
 REPEAT 1020 1108 CSPG 6.
 REPEAT 1128 1216 CSPG 7.
 REPEAT 1241 1337 CSPG 8.
 REPEAT 1360 1449 CSPG 9.
 REPEAT 1476 1561 CSPG 10.
 REPEAT 1583 1679 CSPG 11.
 REPEAT 1707 1803 CSPG 12.
 REPEAT 1835 1922 CSPG 13.
 REPEAT 1944 2029 CSPG 14.
 REPEAT 2048 2147 CSPG 15.
 REGION 30 639 Globular or compact configuration
 REGION 30 639 Neurite growth inhibition (By
 REGION 574 1040 Interaction with COL6A2 (By similarity).
 REGION 631 1446 Interaction with COL5A1 (By similarity).
 REGION 1586 2221 Neurite growth inhibition (By
 REGION 1587 2221 Cysteine-containing.
 MOTIF 2320 2322 PDZ-binding.
 MOD_RES 2252 2252 Phosphothreonine; by PKC/PRKCA (By
 CARBOHYD 130 130 N-linked (GlcNAc...) (Potential).
 CARBOHYD 348 348 N-linked (GlcNAc...) (Potential).
 CARBOHYD 427 427 N-linked (GlcNAc...) (Potential).
 CARBOHYD 685 685 N-linked (GlcNAc...) (Potential).
 CARBOHYD 772 772 N-linked (GlcNAc...) (Potential).
 CARBOHYD 995 995 O-linked (Xyl...) (chondroitin sulfate)
 CARBOHYD 1131 1131 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1202 1202 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1364 1364 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1449 1449 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1645 1645 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1909 1909 N-linked (GlcNAc...) (Potential).
 CARBOHYD 2016 2016 N-linked (GlcNAc...) (Potential).
 CARBOHYD 2034 2034 N-linked (GlcNAc...) (Potential).
 CARBOHYD 2040 2040 N-linked (GlcNAc...) (Potential).
 CARBOHYD 2075 2075 N-linked (GlcNAc...).
 DISULFID 169 192 By similarity.
 DISULFID 354 380 By similarity.  
Keyword
 Angiogenesis; Cell membrane; Cell projection; Complete proteome; Developmental protein; Differentiation; Disulfide bond; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Proteoglycan; Reference proteome; Repeat; Signal; Tissue remodeling; Transducer; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2322 AA 
Protein Sequence
MQSGPRPPLP APGLALALTL TMLARLASAA SFFGENHLEV PVATALTDID LQLQFSTSQP 60
EALLLLAAGP ADHLLLQLYS GRLQVRLVLG QEELRLQTPA ETLLSDSIPH TVVLTVVEGW 120
ATLSVDGFLN ASSAVPGAPL EVPYGLFVGG TGTLGLPYLR GTSRPLRGCL HAATLNGRSL 180
LRPLTPDVHE GCAEEFSASD DVALGFSGPH SLAAFPAWGT QDEGTLEFTL TTQSRQAPLA 240
FQAGGRRGDF IYVDIFEGHL RAVVEKGQGT VLLHNSVPVA DGQPHEVSVH INAHRLEISV 300
DQYPTHTSNR GVLSYLEPRG SLLLGGLDAE ASRHLQEHRL GLTPEATNAS LLGCMEDLSV 360
NGQRRGLREA LLTRNMAAGC RLEEEEYEDD AYGHYEAFST LAPEAWPAME LPEPCVPEPG 420
LPPVFANFTQ LLTISPLVVA EGGTAWLEWR HVQPTLDLME AELRKSQVLF SVTRGARHGE 480
LELDIPGAQA RKMFTLLDVV NRKARFIHDG SEDTSDQLVL EVSVTARVPM PSCLRRGQTY 540
LLPIQVNPVN DPPHIIFPHG SLMVILEHTQ KPLGPEVFQA YDPDSACEGL TFQVLGTSSG 600
LPVERRDQPG EPATEFSCRE LEAGSLVYVH RGGPAQDLTF RVSDGLQASP PATLKVVAIR 660
PAIQIHRSTG LRLAQGSAMP ILPANLSVET NAVGQDVSVL FRVTGALQFG ELQKQGAGGV 720
EGAEWWATQA FHQRDVEQGR VRYLSTDPQH HAYDTVENLA LEVQVGQEIL SNLSFPVTIQ 780
RATVWMLRLE PLHTQNTQQE TLTTAHLEAT LEEAGPSPPT FHYEVVQAPR KGNLQLQGTR 840
LSDGQGFTQD DIQAGRVTYG ATARASEAVE DTFRFRVTAP PYFSPLYTFP IHIGGDPDAP 900
VLTNVLLVVP EGGEGVLSAD HLFVKSLNSA SYLYEVMERP RHGRLAWRGT QDKTTMVTSF 960
TNEDLLRGRL VYQHDDSETT EDDIPFVATR QGESSGDMAW EEVRGVFRVA IQPVNDHAPV 1020
QTISRIFHVA RGGRRLLTTD DVAFSDADSG FADAQLVLTR KDLLFGSIVA VDEPTRPIYR 1080
FTQEDLRKRR VLFVHSGADR GWIQLQVSDG QHQATALLEV QASEPYLRVA NGSSLVVPQG 1140
GQGTIDTAVL HLDTNLDIRS GDEVHYHVTA GPRWGQLVRA GQPATAFSQQ DLLDGAVLYS 1200
HNGSLSPRDT MAFSVEAGPV HTDATLQVTI ALEGPLAPLK LVRHKKIYVF QGEAAEIRRD 1260
QLEAAQEAVP PADIVFSVKS PPSAGYLVMV SRGALADEPP SLDPVQSFSQ EAVDTGRVLY 1320
LHSRPEAWSD AFSLDVASGL GAPLEGVLVE LEVLPAAIPL EAQNFSVPEG GSLTLAPPLL 1380
RVSGPYFPTL LGLSLQVLEP PQHGALQKED GPQARTLSAF SWRMVEEQLI RYVHDGSETL 1440
TDSFVLMANA SEMDRQSHPV AFTVTVLPVN DQPPILTTNT GLQMWEGATA PIPAEALRST 1500
DGDSGSEDLV YTIEQPSNGR VVLRGAPGTE VRSFTQAQLD GGLVLFSHRG TLDGGFRFRL 1560
SDGEHTSPGH FFRVTAQKQV LLSLKGSQTL TVCPGSVQPL SSQTLRASSS AGTDPQLLLY 1620
RVVRGPQLGR LFHAQQDSTG EALVNFTQAE VYAGNILYEH EMPPEPFWEA HDTLELQLSS 1680
PPARDVAATL AVAVSFEAAC PQRPSHLWKN KGLWVPEGQR ARITVAALDA SNLLASVPSP 1740
QRSEHDVLFQ VTQFPSRGQL LVSEEPLHAG QPHFLQSQLA AGQLVYAHGG GGTQQDGFHF 1800
RAHLQGPAGA SVAGPQTSEA FAITVRDVNE RPPQPQASVP LRLTRGSRAP ISRAQLSVVD 1860
PDSAPGEIEY EVQRAPHNGF LSLVGGGLGP VTRFTQADVD SGRLAFVANG SSVAGIFQLS 1920
MSDGASPPLP MSLAVDILPS AIEVQLRAPL EVPQALGRSS LSQQQLRVVS DREEPEAAYR 1980
LIQGPQYGHL LVGGRPTSAF SQFQIDQGEV VFAFTNFSSS HDHFRVLALA RGVNASAVVN 2040
VTVRALLHVW AGGPWPQGAT LRLDPTVLDA GELANRTGSV PRFRLLEGPR HGRVVRVPRA 2100
RTEPGGSQLV EQFTQQDLED GRLGLEVGRP EGRAPGPAGD SLTLELWAQG VPPAVASLDF 2160
ATEPYNAARP YSVALLSVPE AARTEAGKPE SSTPTGEPGP MASSPEPAVA KGGFLSFLEA 2220
NMFSVIIPMC LVLLLLALIL PLLFYLRKRN KTGKHDVQVL TAKPRNGLAG DTETFRKVEP 2280
GQAIPLTAVP GQGPPPGGQP DPELLQFCRT PNPALKNGQY WV 2322 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
 GO:0009986; C:cell surface; TAS:UniProtKB.
 GO:0005576; C:extracellular region; TAS:Reactome.
 GO:0005796; C:Golgi lumen; TAS:Reactome.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
 GO:0043202; C:lysosomal lumen; TAS:Reactome.
 GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
 GO:0000187; P:activation of MAPK activity; IEA:Compara.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0030206; P:chondroitin sulfate biosynthetic process; TAS:Reactome.
 GO:0030207; P:chondroitin sulfate catabolic process; TAS:Reactome.
 GO:0030208; P:dermatan sulfate biosynthetic process; TAS:Reactome.
 GO:0008347; P:glial cell migration; IEA:Compara.
 GO:0035556; P:intracellular signal transduction; IDA:UniProtKB.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:UniProtKB.
 GO:0048771; P:tissue remodeling; IEA:UniProtKB-KW.
 GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; IEA:Compara. 
Interpro
 IPR008985; ConA-like_lec_gl_sf.
 IPR013320; ConA-like_subgrp.
 IPR027210; Cspg4.
 IPR001791; Laminin_G. 
Pfam
 PF02210; Laminin_G_2 
SMART
 SM00282; LamG 
PROSITE
 PS50025; LAM_G_DOMAIN 
PRINTS