CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031143
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein transport protein Sec61 subunit alpha isoform 1 
Protein Synonyms/Alias
 cDNA FLJ59739, highly similar to Protein transport protein Sec61 subunit alpha isoform 1 
Gene Name
 SEC61A1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16VKFLEVIKPFCVILPubiquitination[1, 2, 3, 4, 5]
27VILPEIQKPERKIQFubiquitination[1, 2, 3, 4, 5, 6]
113IEVGDTPKDRALFNGubiquitination[2]
274FRVDLPIKSARYRGQubiquitination[2, 3, 7]
394EVSGSSAKDVAKQLKubiquitination[1, 2, 3, 5, 7]
398SSAKDVAKQLKEQQMubiquitination[1, 2, 3, 5, 7]
401KDVAKQLKEQQMVMRubiquitination[1, 2, 3, 4, 5, 6, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Membrane; Protein transport; Reference proteome; Translocation; Transmembrane; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 482 AA 
Protein Sequence
MTQTARVLVK FLEVIKPFCV ILPEIQKPER KIQFKEKVLW TAITLFIFLV CCQIPLFGIM 60
SSDSADPFYW MRVILASNRG TLMELGISPI VTSGLIMQLL AGAKIIEVGD TPKDRALFNG 120
AQKLFGMIIT IGQSIVYVMT GMYGDPSEMG AGICLLITIQ LFVAGLIVLL LDELLQKGYG 180
LGSGISLFIA TNICETIVWK AFSPTTVNTG RGMEFEGAII ALFHLLATRT DKVRALREAF 240
YRQNLPNLMN LIATIFVFAV VIYFQGFRVD LPIKSARYRG QYNTYPIKLF YTSNIPIILQ 300
SALVSNLYVI SQMLSARFSG NLLVSLLGTW SDTSSGGPAR AYPVGGLCYY LSPPESFGSV 360
LEDPVHAVVY IVFMLGSCAF FSKTWIEVSG SSAKDVAKQL KEQQMVMRGH RETSMVHELN 420
RYIPTAAAFG GLCIGALSVL ADFLGAIGSG TGILLAVTII YQYFEIFVKE QSEVGSMGAL 480
LF 482 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR002208; SecY/SEC61-alpha.
 IPR023201; SecY_su_dom.
 IPR019561; Translocon_Sec61/SecY_plug_dom. 
Pfam
 PF10559; Plug_translocon
 PF00344; SecY 
SMART
  
PROSITE
 PS00755; SECY_1
 PS00756; SECY_2 
PRINTS