CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020211
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cation transport regulator-like protein 1 
Protein Synonyms/Alias
 Blocks Notch protein; Botch 
Gene Name
 CHAC1 
Gene Synonyms/Alias
 BOTCH 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
151EQVSKALKYLNVREAubiquitination[1, 2, 3, 4]
166VLGGYDTKEVTFYPQubiquitination[3, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos. May also act as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. 
Sequence Annotation
  
Keyword
 Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Golgi apparatus; Neurogenesis; Notch signaling pathway; Reference proteome; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 264 AA 
Protein Sequence
MGGAQLELPS GARPGVCVRR SFRAHAGDQP RRPPGPIPVP GTMKQESAAP NTPPTSQSPT 60
PSAQFPRNDG DPQALWIFGY GSLVWRPDFA YSDSRVGFVR GYSRRFWQGD TFHRGSDKMP 120
GRVVTLLEDH EGCTWGVAYQ VQGEQVSKAL KYLNVREAVL GGYDTKEVTF YPQDAPDQPL 180
KALAYVATPQ NPGYLGPAPE EAIATQILAC RGFSGHNLEY LLRLADFMQL CGPQAQDEHL 240
AAIVDAVGTM LPCFCPTEQA LALV 264 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
 GO:0005112; F:Notch binding; ISS:UniProtKB.
 GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; IMP:UniProtKB.
 GO:0045746; P:negative regulation of Notch signaling pathway; IMP:UniProtKB.
 GO:0010955; P:negative regulation of protein processing; IMP:UniProtKB.
 GO:0022008; P:neurogenesis; ISS:UniProtKB.
 GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW. 
Interpro
 IPR013024; Butirosin_synth_BtrG-like.
 IPR006840; ChaC. 
Pfam
 PF04752; ChaC 
SMART
  
PROSITE
  
PRINTS