CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040732
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nck-associated protein 1-like 
Protein Synonyms/Alias
  
Gene Name
 NCKAP1L 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10LTSAYQHKLAEKLTIubiquitination[1, 2]
14YQHKLAEKLTILNDRubiquitination[1, 2, 3, 4]
33LIRMYNIKKTCSDPKubiquitination[1]
40KKTCSDPKSKPPFLLubiquitination[1, 2]
42TCSDPKSKPPFLLEKubiquitination[1, 2]
49KPPFLLEKSMEPSLKubiquitination[1, 2]
56KSMEPSLKYINKKFPubiquitination[1, 2, 5]
60PSLKYINKKFPNIDVubiquitination[1, 3, 5]
61SLKYINKKFPNIDVRubiquitination[1, 3, 5]
81LGPVHREKAEIIRFLubiquitination[1]
188LEYDHPLKKLTEEFGubiquitination[1]
189EYDHPLKKLTEEFGPubiquitination[1]
313EDLFSSLKGYGKRVAubiquitination[1, 2]
323GKRVADIKESKEHVIubiquitination[1]
326VADIKESKEHVIANSubiquitination[1, 2]
349QFLRMAVKELETVLAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 449 AA 
Protein Sequence
MSLTSAYQHK LAEKLTILND RGQGVLIRMY NIKKTCSDPK SKPPFLLEKS MEPSLKYINK 60
KFPNIDVRNS TQHLGPVHRE KAEIIRFLTN YYQSFVDVME FRDHVYELLN TIDACQCHFD 120
INLNFDFTRS YLDLIVTYTS VILLLSRIED RRILIGMYNC AHEMLHGHGD PSFARLGQMV 180
LEYDHPLKKL TEEFGPHTKA VSGALLSLHF LFVRRNQGAE QWRSAQLLSL ISNPPAMINP 240
ANSDTMACEY LSVEVMERWI IIGFLLCHGC LNSNSQCQKL WKLCLQGSLY ITLIREDVLQ 300
VHKVTEDLFS SLKGYGKRVA DIKESKEHVI ANSGQFHCQR RQFLRMAVKE LETVLADEPG 360
LLGPKALFAF MALSFIRDEV TWLVRHTENV TKTKTPEDYA DSSIAELLFL LEGIRSLVRR 420
HIKVIQQYHL QYLARFDALV LSDIIQFLS 449 
Gene Ontology
  
Interpro
 IPR019137; Nck-associated_protein-1. 
Pfam
 PF09735; Nckap1 
SMART
  
PROSITE
  
PRINTS