UniProt Accession

 THTR_RAT; P24329; Q5I0D4 

Genbank Protein ID

 BC088449; X56228 

Genbank Nucleotide ID

 AAH88449.1; CAA39677.1 

Protein Name

 Thiosulfate sulfurtransferase 

Protein Synonyms/Alias

 Rhodanese 

Gene Name

 Tst 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
14	YRALVSTKWLAESIR	acetylation	[1]
24	AESIRSGKVGPSLRV	acetylation	[1]
136	GGFRNWLKEGHPVTS	acetylation	[1]
154	RPEPAVFKATLNRSL	acetylation	[1]
163	TLNRSLLKTYEQVLE	acetylation	[1]
175	VLENLQSKRFQLVDS	acetylation	[1]
236	LRAIFQDKKVDLSQP	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA (By similarity). Involved in the formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. 

Sequence Annotation

 DOMAIN 25 143 Rhodanese 1.
 DOMAIN 173 288 Rhodanese 2.
 REGION 144 159 Hinge.
 ACT_SITE 248 248 Cysteine persulfide intermediate (By
 BINDING 187 187 Substrate (By similarity).
 BINDING 250 250 Substrate (By similarity).
 MOD_RES 14 14 N6-acetyllysine; alternate (By
 MOD_RES 14 14 N6-succinyllysine; alternate (By
 MOD_RES 136 136 N6-acetyllysine (By similarity).
 MOD_RES 164 164 Phosphothreonine (By similarity).
 MOD_RES 165 165 Phosphotyrosine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Mitochondrion; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 297 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0008097; F:5S rRNA binding; ISS:UniProtKB.
 GO:0004792; F:thiosulfate sulfurtransferase activity; IEA:EC.
 GO:0016226; P:iron-sulfur cluster assembly; NAS:RGD.
 GO:0035928; P:rRNA import into mitochondrion; ISS:UniProtKB. 

Interpro

 IPR001763; Rhodanese-like_dom.
 IPR001307; Thiosulphate_STrfase_CS. 

Pfam

 PF00581; Rhodanese 

SMART

 SM00450; RHOD 

PROSITE

 PS00380; RHODANESE_1
 PS00683; RHODANESE_2
 PS50206; RHODANESE_3 

PRINTS