UniProt Accession

 NUD16_MOUSE; Q6P3D0; Q9D716 

Genbank Protein ID

 AK009737; BC064048 

Genbank Nucleotide ID

 BAB26469.1; AAH64048.1 

Protein Name

 U8 snoRNA-decapping enzyme 

Protein Synonyms/Alias

 IDP phosphatase; IDPase; Inosine diphosphate phosphatase; Nucleoside diphosphate-linked moiety X motif 16; Nudix motif 16; m7GpppN-mRNA hydrolase 

Gene Name

 Nudt16 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
188	LDPGIIAKLKIPDSK	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal- dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms. The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP. Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. 

Sequence Annotation

 DOMAIN 18 173 Nudix hydrolase.
 MOTIF 61 82 Nudix box.
 METAL 59 59 Magnesium or manganese 1; via carbonyl
 METAL 76 76 Magnesium or manganese 2 (By similarity).
 METAL 76 76 Magnesium or manganese 3 (By similarity).
 METAL 80 80 Magnesium or manganese 1 (By similarity).
 METAL 80 80 Magnesium or manganese 3 (By similarity).
 METAL 136 136 Magnesium or manganese 3 (By similarity).
 METAL 136 136 Magnesium or manganese 4 (By similarity).
 BINDING 24 24 Substrate (By similarity).
 BINDING 50 50 Substrate (By similarity).
 BINDING 170 170 Substrate (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Hydrolase; Magnesium; Manganese; Metal-binding; Nucleotide metabolism; Nucleotide-binding; Nucleus; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 195 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0050897; F:cobalt ion binding; ISS:UniProtKB.
 GO:0035870; F:dITP diphosphatase activity; IMP:MGI.
 GO:0005525; F:GTP binding; ISS:UniProtKB.
 GO:1901641; F:ITP binding; ISS:UniProtKB.
 GO:0050072; F:m7G(5')pppN diphosphatase activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0030145; F:manganese ion binding; ISS:UniProtKB.
 GO:0008235; F:metalloexopeptidase activity; ISS:UniProtKB.
 GO:0003729; F:mRNA binding; ISS:UniProtKB.
 GO:0019204; F:nucleotide phosphatase activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0030515; F:snoRNA binding; ISS:UniProtKB.
 GO:1901640; F:XTP binding; ISS:UniProtKB.
 GO:0006382; P:adenosine to inosine editing; ISS:UniProtKB.
 GO:0051276; P:chromosome organization; IGI:MGI.
 GO:0035863; P:dITP catabolic process; IGI:MGI.
 GO:0046709; P:IDP catabolic process; ISS:UniProtKB.
 GO:0006402; P:mRNA catabolic process; IMP:UniProtKB.
 GO:2000233; P:negative regulation of rRNA processing; ISS:UniProtKB.
 GO:0090068; P:positive regulation of cell cycle process; ISS:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; ISS:UniProtKB.
 GO:2000781; P:positive regulation of double-strand break repair; ISS:UniProtKB.
 GO:0016077; P:snoRNA catabolic process; ISS:UniProtKB.
 GO:1901639; P:XDP catabolic process; ISS:UniProtKB. 

Interpro

 IPR000086; NUDIX_hydrolase_dom.
 IPR015797; NUDIX_hydrolase_dom-like. 

Pfam

 PF00293; NUDIX 

SMART

  

PROSITE

 PS51462; NUDIX
 PS00893; NUDIX_BOX 

PRINTS