CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011982
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional coenzyme A synthase 
Protein Synonyms/Alias
 CoA synthase; NBP; POV-2; Phosphopantetheine adenylyltransferase; Dephospho-CoA pyrophosphorylase; Pantetheine-phosphate adenylyltransferase; PPAT; Dephospho-CoA kinase; DPCK; Dephosphocoenzyme A kinase; DPCOAK 
Gene Name
 COASY 
Gene Synonyms/Alias
 PSEC0106 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
264DKDLLKSKLLPELLQubiquitination[1]
359HTENEEDKVSSSSFRubiquitination[2]
408SSIAQRLKGLGAFVIubiquitination[2]
583KAWALLQKRIPKTHQubiquitination[1]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. 
Sequence Annotation
 DOMAIN 360 563 DPCK.
 NP_BIND 365 372 ATP (Potential).
 REGION 180 358 Phosphopantetheine adenylyltransferase.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 183 183 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Coenzyme A biosynthesis; Complete proteome; Cytoplasm; Kinase; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 564 AA 
Protein Sequence
MRTPRLRAQP RGAVYQAPSP PPAPVGLGSM AVFRSGLLVL TTPLASLAPR LASILTSAAR 60
LVNHTLYVHL QPGMSLEGPA QPQSSPVQAT FEVLDFITHL YAGADVHRHL DVRILLTNIR 120
TKSTFLPPLP TSVQNLAHPP EVVLTDFQTL DGSQYNPVKQ QLVRYATSCY SCCPRLASVL 180
LYSDYGIGEV PVEPLDVPLP STIRPASPVA GSPKQPVRGY YRGAVGGTFD RLHNAHKVLL 240
SVACILAQEQ LVVGVADKDL LKSKLLPELL QPYTERVEHL SEFLVDIKPS LTFDVIPLLD 300
PYGPAGSDPS LEFLVVSEET YRGGMAINRF RLENDLEELA LYQIQLLKDL RHTENEEDKV 360
SSSSFRQRML GNLLRPPYER PELPTCLYVI GLTGISGSGK SSIAQRLKGL GAFVIDSDHL 420
GHRAYAPGGP AYQPVVEAFG TDILHKDGII NRKVLGSRVF GNKKQLKILT DIMWPIIAKL 480
AREEMDRAVA EGKRVCVIDA AVLLEAGWQN LVHEVWTAVI PETEAVRRIV ERDGLSEAAA 540
QSRLQSQMSG QQLVEQSHVV LSTLWEPHIT QRQVEKAWAL LQKRIPKTHQ ALD 593 
Gene Ontology
 GO:0005741; C:mitochondrial outer membrane; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004140; F:dephospho-CoA kinase activity; IDA:UniProtKB.
 GO:0004595; F:pantetheine-phosphate adenylyltransferase activity; IDA:UniProtKB.
 GO:0015937; P:coenzyme A biosynthetic process; IDA:UniProtKB.
 GO:0015939; P:pantothenate metabolic process; TAS:Reactome. 
Interpro
 IPR004821; Cyt_trans-like.
 IPR001977; Depp_CoAkinase.
 IPR027417; P-loop_NTPase.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF01121; CoaE
 PF01467; CTP_transf_2 
SMART
  
PROSITE
 PS51219; DPCK 
PRINTS