CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044058
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Protein diaphanous homolog 1 
Protein Synonyms/Alias
  
Gene Name
 DIAPH1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
133SQYLYTSKAGMSQKEubiquitination[1]
202LKRLHDEKEETAGSYubiquitination[1]
222HEIIRCLKAFMNNKFubiquitination[1]
303QPLLDGLKSGTTIALubiquitination[1]
377EEDSYDLKGRLDDIRubiquitination[1]
453NGADPDFKCRHLQIEubiquitination[1]
487KAAELEKKLDSELTAubiquitination[1]
503HELQVEMKKMESDFEubiquitination[1]
504ELQVEMKKMESDFEQubiquitination[1]
771PFGLTPKKLYKPEVQubiquitination[1]
774LTPKKLYKPEVQLRRubiquitination[1]
786LRRPNWSKLVAEDLSubiquitination[1]
800SQDCFWTKVKEDRFEubiquitination[1]
802DCFWTKVKEDRFENNubiquitination[1]
856ELKVLDSKTAQNLSIubiquitination[1]
1225GPRQANRKAGCAVTSubiquitination[1]
1240LLASELTKDDAMAAVubiquitination[1]
1253AVPAKVSKNSETFPTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1273 AA 
Protein Sequence
MEPPGGSLGP GRGTRDKKKG RSPDELPSAG GDGGKSKKFT LKRLMADELE RFTSMRIKKE 60
KEKPNSAHRN SSASYGDDPT AQSLQDVSDE QVLVLFEQML LDMNLNEEKQ QPLREKDIII 120
KREMVSQYLY TSKAGMSQKE SSKSAMMYIQ ELRSGLRDMP LLSCLESLRV SLNNNPVSWV 180
QTFGAEGLAS LLDILKRLHD EKEETAGSYD SRNKHEIIRC LKAFMNNKFG IKTMLETEEG 240
ILLLVRAMDP AVPNMMIDAA KLLSALCILP QPEDMNERVL EAMTERAEMD EVERFQPLLD 300
GLKSGTTIAL KVGCLQLINA LITPAEELDF RVHIRSELMR LGLHQVLQDL REIENEDMRV 360
QLNVFDEQGE EDSYDLKGRL DDIRMEMDDF NEVFQILLNT VKDSKAEPHF LSILQHLLLV 420
RNDYEARPQY YKLIEECISQ IVLHKNGADP DFKCRHLQIE IEGLIDQMID KTKVEKSEAK 480
AAELEKKLDS ELTARHELQV EMKKMESDFE QKLQDLQGEK DALHSEKQQI ATEKQDLEAE 540
VSQLTGEVAK LTKELEDAKK EMASLSAAAI TVPPSVPSRA PVPPAPPLPG DSGTIIPPPP 600
APGDSTTPPP PPPPPPPPPP LPGGVCISSP PSLPGGTAIS PPPPLSGDAT IPPPPPLPEG 660
VGIPSPSSLP GGTAIPPPPP LPGSARIPPP PPPLPGSAGI PPPPPPLPGE AGMPPPPPPL 720
PGGPGIPPPP PFPGGPGIPP PPPGMGMPPP PPFGFGVPAA PVLPFGLTPK KLYKPEVQLR 780
RPNWSKLVAE DLSQDCFWTK VKEDRFENNE LFAKLTLTFS AQTKRTKKAK KDQEGGEEKK 840
SVQKKKVKEL KVLDSKTAQN LSIFLGSFRM PYQEIKNVIL EVNEAVLTES MIQNLIKQMP 900
EPEQLKMLSE LKDEYDDLAE SEQFGVVMGT VPRLRPRLNA ILFKLQFSEQ VENIKPEIVS 960
VTAACEELRK SESFSNLLEI TLLVGNYMNA GSRNAGAFGF NISFLCKLRD TKSTDQKMTL 1020
LHFLAELCEN DYPDVLKFPD ELAHVEKASR VSAENLQKNL DQMKKQISDV ERDVQNFPAA 1080
TDEKDKFVEK MTIFVKDAQE QYNKLRMMHS NMETLYKELG EYFLFDPKKL SVEEFFMDLH 1140
NFRNMFLQAV KENQKRRETE EKMRRAKLAK EKAEKERLEK QQKREQLIDM NAEGDETGVM 1200
DSLLEALQSG AAFRRKRGPR QANRKAGCAV TSLLASELTK DDAMAAVPAK VSKNSETFPT 1260
ILEEAKELVG RAS 1273 
Gene Ontology
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro. 
Interpro
 IPR003104; Actin-bd_FH2/DRF_autoreg.
 IPR016024; ARM-type_fold.
 IPR014767; Diaphanous_autoregulatory.
 IPR010465; Drf_DAD.
 IPR010472; Drf_FH3.
 IPR010473; Drf_GTPase-bd.
 IPR015425; FH2_actin-bd.
 IPR009408; Formin_homology_1.
 IPR014768; GTPase-bd/formin_homology_3. 
Pfam
 PF06345; Drf_DAD
 PF06346; Drf_FH1
 PF06367; Drf_FH3
 PF06371; Drf_GBD
 PF02181; FH2 
SMART
 SM00498; FH2 
PROSITE
 PS51231; DAD
 PS51444; FH2
 PS51232; GBD_FH3 
PRINTS