UniProt Accession

 FOLD_ECOLI; P24186; P77132; Q2MBQ0 

Genbank Protein ID

 M74789; D10588; U82664; U00096; AP009048 

Genbank Nucleotide ID

 AAA23803.1; BAA01445.1; AAB40282.1; AAC73631.1; BAE76306.1 

Protein Name

 Bifunctional protein FolD 

Protein Synonyms/Alias

 Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase 

Gene Name

 folD 

Gene Synonyms/Alias

 ads; b0529; JW0518 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
4	****MAAKIIDGKTI	acetylation	[1]
9	AAKIIDGKTIAQQVR	acetylation	[1]
54	SQIYVASKRKACEEV	acetylation	[1]
118	LERIHPDKDVDGFHP	acetylation	[1]
239	INRLENGKVVGDVVF	acetylation	[1]
251	VVFEDAAKRASYITP	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate. This enzyme is specific for NADP. 

Sequence Annotation

  

Keyword

 3D-structure; Amino-acid biosynthesis; Complete proteome; Direct protein sequencing; Histidine biosynthesis; Hydrolase; Methionine biosynthesis; Multifunctional enzyme; NADP; One-carbon metabolism; Oxidoreductase; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 288 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IBA:RefGenome.
 GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IBA:RefGenome.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
 GO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
 GO:0006730; P:one-carbon metabolic process; IBA:RefGenome.
 GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 

Interpro

 IPR016040; NAD(P)-bd_dom.
 IPR000672; THF_DH/CycHdrlase.
 IPR020630; THF_DH/CycHdrlase_cat_dom.
 IPR020867; THF_DH/CycHdrlase_CS.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 

Pfam

 PF00763; THF_DHG_CYH
 PF02882; THF_DHG_CYH_C 

SMART

  

PROSITE

 PS00766; THF_DHG_CYH_1
 PS00767; THF_DHG_CYH_2 

PRINTS

 PR00085; THFDHDRGNASE.