UniProt Accession

 ODPA_MOUSE; P35486 

Genbank Protein ID

 M76727; BC007142 

Genbank Nucleotide ID

 AAA53046.1; AAH07142.1 

Protein Name

 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial 

Protein Synonyms/Alias

 PDHE1-A type I 

Gene Name

 Pdha1 

Gene Synonyms/Alias

 Pdha-1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
39	NDATFEIKKCDLHRL	acetylation	[1]
63	LTREDGLKYYRMMQT	acetylation	[1, 2, 3, 4]
63	LTREDGLKYYRMMQT	succinylation	[3]
77	TVRRMELKADQLYKQ	acetylation	[1, 2, 3, 4, 5, 6, 7]
77	TVRRMELKADQLYKQ	succinylation	[3]
83	LKADQLYKQKIIRGF	acetylation	[1, 2, 3, 4, 5]
83	LKADQLYKQKIIRGF	succinylation	[3]
244	AASTDYYKRGDFIPG	acetylation	[1, 2, 3, 4]
244	AASTDYYKRGDFIPG	succinylation	[3]
267	LCVREATKFAAAYCR	acetylation	[1, 2, 4, 6]
277	AAYCRSGKGPILMEL	acetylation	[3]
277	AAYCRSGKGPILMEL	succinylation	[3]
313	EIQEVRSKSDPIMLL	acetylation	[1, 2, 3, 4, 6]
313	EIQEVRSKSDPIMLL	succinylation	[3]
321	SDPIMLLKDRMVNSN	acetylation	[1, 2, 3, 4, 5, 6, 7, 8, 9, 10]
321	SDPIMLLKDRMVNSN	succinylation	[3]
321	SDPIMLLKDRMVNSN	ubiquitination	[11]
385	RGANQWIKFKSVS**	acetylation	[1, 2, 3]
385	RGANQWIKFKSVS**	succinylation	[3]
385	RGANQWIKFKSVS**	ubiquitination	[11]

Reference

 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [6] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [8] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [9] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [10] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [11] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. 

Sequence Annotation

 MOD_RES 232 232 Phosphoserine; by PDK1.
 MOD_RES 289 289 Phosphotyrosine.
 MOD_RES 293 293 Phosphoserine; by PDK1, PDK2, PDK3 and
 MOD_RES 300 300 Phosphoserine; by PDK1, PDK2, PDK3 and
 MOD_RES 301 301 Phosphotyrosine.
 MOD_RES 321 321 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Glucose metabolism; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate; Reference proteome; Thiamine pyrophosphate; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 390 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0045254; C:pyruvate dehydrogenase complex; ISS:UniProtKB.
 GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IEA:EC.
 GO:0004738; F:pyruvate dehydrogenase activity; IMP:MGI.
 GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; ISS:UniProtKB.
 GO:0006096; P:glycolysis; IEA:InterPro.
 GO:0006099; P:tricarboxylic acid cycle; ISS:UniProtKB. 

Interpro

 IPR001017; DH_E1.
 IPR017597; Pyrv_DH_E1_asu_subgrp-y. 

Pfam

 PF00676; E1_dh 

SMART

  

PROSITE

  

PRINTS