CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011182
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UBX domain-containing protein 1 
Protein Synonyms/Alias
 SAPK substrate protein 1; UBA/UBX 33.3 kDa protein 
Gene Name
 UBXN1 
Gene Synonyms/Alias
 SAKS1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
83GSAAGEGKPALSEEEubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
96EERQEQTKRMLELVAubiquitination[3, 7]
105MLELVAQKQREREERubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
154AEERRREKAEELAARubiquitination[2, 3, 5, 6, 7]
178DKAERAKKYGGSVGSubiquitination[2, 3, 6, 7]
208PSQEPPTKREYDQCRubiquitination[3, 6, 7]
293SAVLIVAKKCPS***ubiquitination[3, 7]
294AVLIVAKKCPS****ubiquitination[6]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Ubiquitin-binding protein that interacts with the BRCA1- BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1, leads to inhibit the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. 
Sequence Annotation
 DOMAIN 1 42 UBA.
 DOMAIN 211 293 UBX.
 REGION 43 297 Interaction with BRCA1.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 199 199 Phosphoserine.
 MOD_RES 200 200 Phosphoserine; by MAPK12.
 MOD_RES 270 270 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 297 AA 
Protein Sequence
MAELTALESL IEMGFPRGRA EKALALTGNQ GIEAAMDWLM EHEDDPDVDE PLETPLGHIL 60
GREPTSSEQG GLEGSGSAAG EGKPALSEEE RQEQTKRMLE LVAQKQRERE EREEREALER 120
ERQRRRQGQE LSAARQRLQE DEMRRAAEER RREKAEELAA RQRVREKIER DKAERAKKYG 180
GSVGSQPPPV APEPGPVPSS PSQEPPTKRE YDQCRIQVRL PDGTSLTQTF RAREQLAAVR 240
LYVELHRGEE LGGGQDPVQL LSGFPRRAFS EADMERPLQE LGLVPSAVLI VAKKCPS 297 
Gene Ontology
 GO:0034098; C:Cdc48p-Npl4p-Ufd1p AAA ATPase complex; IDA:BHF-UCL.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0071796; F:K6-linked polyubiquitin binding; IDA:UniProtKB.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:BHF-UCL.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0031397; P:negative regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; NAS:UniProtKB. 
Interpro
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk.
 IPR001012; UBX. 
Pfam
 PF00627; UBA
 PF00789; UBX 
SMART
 SM00165; UBA
 SM00166; UBX 
PROSITE
 PS50030; UBA
 PS50033; UBX 
PRINTS