CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040807
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 NEDD8-activating enzyme E1 catalytic subunit 
Protein Synonyms/Alias
  
Gene Name
 UBA3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
83PKDIGRPKAEVAAEFubiquitination[1]
106NVVPHFNKIQDFNDTubiquitination[1, 2, 3, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 422 AA 
Protein Sequence
MADGEEPMAV DGGCGDTGDW EGRWNHVKKF LERSGPFTHP DFEPSTEALS GFRQIHVIDM 60
DTIDVSNLNR QFLFRPKDIG RPKAEVAAEF LNDRVPNCNV VPHFNKIQDF NDTFYRQFHI 120
IVCGLDSIIA RRWINGMLIS LLNYEDGVLD PSSIVPLIDG GTEGFKGNAR VILPGMTACI 180
ECTLELYPPQ VNFPMCTIAS MPRLPEHCIE YVRMLQWPKE QPFGEGVPLD GDDPEHIQWI 240
FQKSLERASQ YNIRGVTYRL TQGVVKRIIP AVASTNAVIA AVCATEVFKI ATSAYIPLNN 300
YLVFNDVDGL YTYTFEAERK ENCPACSQLP QNIQFSPSAK LQEVLDYLTN SASLQMKSPA 360
ITATLEGKNR TLYLQSVTSI EERTRPNLSK TLKELGLVDG QELAVADVTT PQTVLFKLHF 420
TS 422 
Gene Ontology
 GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0008641; F:small protein activating enzyme activity; IEA:InterPro.
 GO:0045116; P:protein neddylation; IEA:InterPro. 
Interpro
 IPR014929; E2_binding.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR023318; Ub_act_enz_dom_a.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018074; UBQ-activ_enz_E1_AS. 
Pfam
 PF08825; E2_bind
 PF00899; ThiF
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 
SMART
  
PROSITE
 PS00865; UBIQUITIN_ACTIVAT_2 
PRINTS