CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006223
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cobalt uptake protein COT1 
Protein Synonyms/Alias
  
Gene Name
 COT1 
Gene Synonyms/Alias
 YOR316C; O6131 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
212LTPENASKTPSYSTSubiquitination[1]
301PLSCKASKILLQATPubiquitination[1, 2, 3, 4]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269
Functional Description
 Probably responsible for the uptake of cobalt ions. It appears to act in a dosage-dependent manner to counteract the adverse effects of cobalt ions on cells. It may participate in the regulation of cobalt levels under normal physiological conditions and may be important in the supply of metal that is required for metalloenzyme or cofactor synthesis. It reduces the toxicity of cobalt and rhodium ions. Other components responsible for cobalt transport exist. 
Sequence Annotation
 MOD_RES 225 225 Phosphoserine.
 CROSSLNK 301 301 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Cobalt; Complete proteome; Isopeptide bond; Membrane; Mitochondrion; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 439 AA 
Protein Sequence
MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL VVALWAVNVA 60
KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL QRIIAPPVIE NPKFVLYVGV 120
AGLISNTVGL FLFHDNDQEH GHGHGHSHGG IFADHEMHMP SSHTHTHAHV DGIENTTPMD 180
STDNISEIMP NAIVDSFMNE NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN 240
MHGVFLHVLG DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS 300
KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL DISPEQFTDL 360
AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH LQNDPLSLRP KTYGTGISGS 420
TCLIDDAANC NTADCLEDH 439 
Gene Ontology
 GO:0000324; C:fungal-type vacuole; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0015087; F:cobalt ion transmembrane transporter activity; IMP:SGD.
 GO:0005385; F:zinc ion transmembrane transporter activity; IGI:SGD.
 GO:0006882; P:cellular zinc ion homeostasis; IMP:SGD. 
Interpro
 IPR002524; Cation_efflux.
 IPR027469; Cation_efflux_TMD. 
Pfam
 PF01545; Cation_efflux 
SMART
  
PROSITE
  
PRINTS