CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014226
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Trifunctional enzyme subunit beta, mitochondrial 
Protein Synonyms/Alias
 TP-beta; 3-ketoacyl-CoA thiolase; Acetyl-CoA acyltransferase; Beta-ketothiolase 
Gene Name
 Hadhb 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
53TLAKPNLKNIVVVEGacetylation[1]
73LLSGTSYKDLMPHDLacetylation[1]
189KMMLDLNKAKTLAQRacetylation[1]
202QRLSLLTKFRLNFLSacetylation[1]
244VSRMEQDKYALRSHSacetylation[1]
269LSDIVPFKVPGKDTVacetylation[1]
273VPFKVPGKDTVSKDNacetylation[1]
278PGKDTVSKDNGIRPSacetylation[1]
294LEQMAKLKPAFIKPYacetylation[1]
299KLKPAFIKPYGTVTAacetylation[1]
333RALAMGYKPKAYLRDacetylation[1]
335LAMGYKPKAYLRDFIacetylation[1]
349IYVSQDPKDQLLLGPacetylation[1]
362GPTYATPKVLEKAGLacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 ACT_SITE 139 139 Acyl-thioester intermediate (By
 ACT_SITE 429 429 Proton acceptor (By similarity).
 ACT_SITE 459 459 Proton acceptor (By similarity).
 MOD_RES 73 73 N6-acetyllysine (By similarity).
 MOD_RES 189 189 N6-acetyllysine (By similarity).
 MOD_RES 202 202 N6-acetyllysine (By similarity).
 MOD_RES 349 349 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Acyltransferase; Complete proteome; Endoplasmic reticulum; Fatty acid metabolism; Lipid metabolism; Membrane; Mitochondrion; Mitochondrion inner membrane; Mitochondrion outer membrane; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 475 AA 
Protein Sequence
MTTILTSTFR NLSTTSKWAL RFSVRPLSCS SQVQSAPAVQ TKSKKTLAKP NLKNIVVVEG 60
VRIPFLLSGT SYKDLMPHDL ARAALSGLLY RTNIPKDVVD YIIFGTVIQE VKTSNVAREA 120
ALGAGFSDKT PAHTVTMACI SSNQAMTTAV GLIASGQCDV VVAGGVELMS DVPIRHSRNM 180
RKMMLDLNKA KTLAQRLSLL TKFRLNFLSP ELPAVAEFST NETMGHSADR LAAAFAVSRM 240
EQDKYALRSH SLAKKAQDEG HLSDIVPFKV PGKDTVSKDN GIRPSSLEQM AKLKPAFIKP 300
YGTVTAANSS FLTDGASAML IMSEDRALAM GYKPKAYLRD FIYVSQDPKD QLLLGPTYAT 360
PKVLEKAGLT MNDIDAFEFH EAFSGQILAN FKAMDSDWFA QNYMGRKTKV GAPPLEKFNI 420
WGGSLSLGHP FGATGCRLVM AAANRLRKDG GQYALVAACA AGGQGHAMIV EAYPK 475 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0016507; C:mitochondrial fatty acid beta-oxidation multienzyme complex; IDA:RGD.
 GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
 GO:0042645; C:mitochondrial nucleoid; IEA:Compara.
 GO:0005741; C:mitochondrial outer membrane; ISS:UniProtKB.
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IDA:RGD.
 GO:0003988; F:acetyl-CoA C-acyltransferase activity; IDA:RGD.
 GO:0004300; F:enoyl-CoA hydratase activity; IDA:RGD.
 GO:0000062; F:fatty-acyl-CoA binding; IDA:RGD.
 GO:0016509; F:long-chain-3-hydroxyacyl-CoA dehydrogenase activity; IDA:RGD.
 GO:0016508; F:long-chain-enoyl-CoA hydratase activity; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0032403; F:protein complex binding; IMP:RGD.
 GO:0006635; P:fatty acid beta-oxidation; IDA:RGD. 
Interpro
 IPR002155; Thiolase.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr.
 IPR020615; Thiolase_acyl_enz_int_AS.
 IPR020610; Thiolase_AS.
 IPR020617; Thiolase_C.
 IPR020613; Thiolase_CS.
 IPR020616; Thiolase_N. 
Pfam
 PF02803; Thiolase_C
 PF00108; Thiolase_N 
SMART
  
PROSITE
 PS00098; THIOLASE_1
 PS00737; THIOLASE_2
 PS00099; THIOLASE_3 
PRINTS