CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020020
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein NipSnap homolog 1 
Protein Synonyms/Alias
 NipSnap1 
Gene Name
 NIPSNAP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37AAARFYSKDNEGSWFubiquitination[1, 2, 3]
51FRSLFVHKVDPRKDAacetylation[4]
51FRSLFVHKVDPRKDAubiquitination[5, 6]
65AHSTLLSKKETSNLYubiquitination[5, 6]
66HSTLLSKKETSNLYKubiquitination[5, 6]
80KIQFHNVKPEYLDAYubiquitination[3]
146MNKLKNNKEYLEFRRacetylation[4]
193ELRTYKLKPGTMIEWubiquitination[2, 5, 6]
253TRNAAWRKRGWDENVubiquitination[5, 6]
279SRIMIPLKISPLQ**ubiquitination[2, 3, 7]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 MOD_RES 146 146 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 284 AA 
Protein Sequence
MAPRLCSISV TARRLLGGPG PRAGDVASAA AARFYSKDNE GSWFRSLFVH KVDPRKDAHS 60
TLLSKKETSN LYKIQFHNVK PEYLDAYNSL TEAVLPKLHL DEDYPCSLVG NWNTWYGEQD 120
QAVHLWRFSG GYPALMDCMN KLKNNKEYLE FRRERSQMLL SRRNQLLLEF SFWNEPQPRM 180
GPNIYELRTY KLKPGTMIEW GNNWARAIKY RQENQEAVGG FFSQIGELYV VHHLWAYKDL 240
QSREETRNAA WRKRGWDENV YYTVPLVRHM ESRIMIPLKI SPLQ 284 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0097060; C:synaptic membrane; IEA:Compara.
 GO:0042165; F:neurotransmitter binding; IEA:Compara.
 GO:0019233; P:sensory perception of pain; IEA:Compara. 
Interpro
 IPR011008; Dimeric_a/b-barrel.
 IPR012577; NIPSNAP. 
Pfam
 PF07978; NIPSNAP 
SMART
  
PROSITE
  
PRINTS