UniProt Accession

 AK1D1_MOUSE; Q8VCX1 

Genbank Protein ID

 BC018333 

Genbank Nucleotide ID

 AAH18333.1 

Protein Name

 3-oxo-5-beta-steroid 4-dehydrogenase 

Protein Synonyms/Alias

 Aldo-keto reductase family 1 member D1; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase 

Gene Name

 Akr1d1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
35	DPRPVPGKTYVAVKT	ubiquitination	[1]
163	CKDAGLVKSLGVSNF	ubiquitination	[1]
181	QLELILNKPGLKYKP	ubiquitination	[1]
187	NKPGLKYKPVTNQVE	ubiquitination	[1]
203	HPYFTQTKLLKFCQQ	acetylation	[2]
248	ELLTSLGKKYNKTQA	ubiquitination	[1]
303	KDIDALNKNVRYVEL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7- alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4- cholesten-3-one can also act as substrates. 

Sequence Annotation

 NP_BIND 168 169 NADP (By similarity).
 NP_BIND 219 223 NADP (By similarity).
 NP_BIND 272 282 NADP (By similarity).
 ACT_SITE 57 57 Proton donor (By similarity).
 BINDING 52 52 NADP (By similarity).
 BINDING 192 192 NADP (By similarity).
 BINDING 229 229 Substrate (By similarity).  

Keyword

 Bile acid catabolism; Complete proteome; Cytoplasm; Lipid degradation; Lipid metabolism; NADP; Oxidoreductase; Reference proteome; Steroid metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 325 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0047787; F:delta4-3-oxosteroid 5beta-reductase activity; IEA:EC.
 GO:0008209; P:androgen metabolic process; IEA:Compara.
 GO:0006699; P:bile acid biosynthetic process; IEA:Compara.
 GO:0030573; P:bile acid catabolic process; IEA:UniProtKB-KW.
 GO:0008207; P:C21-steroid hormone metabolic process; IEA:Compara.
 GO:0006707; P:cholesterol catabolic process; IEA:Compara.
 GO:0007586; P:digestion; IEA:Compara. 

Interpro

 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 

PRINTS

 PR00069; ALDKETRDTASE.