UniProt Accession

 TYB4_MOUSE; P20065 

Genbank Protein ID

 X16053; X16053; U38967 

Genbank Nucleotide ID

 CAA34187.1; CAA34188.1; AAC52490.1 

Protein Name

 Thymosin beta-4 

Protein Synonyms/Alias

 T beta 4; Hematopoietic system regulatory peptide; Seraspenide 

Gene Name

 Tmsb4x 

Gene Synonyms/Alias

 Ptmb4; Tmsb4 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
10	LPATMSDKPDMAEIE	acetylation	[1, 2, 3, 4]
10	LPATMSDKPDMAEIE	ubiquitination	[5]
18	PDMAEIEKFDKSKLK	acetylation	[2, 3, 4]
18	PDMAEIEKFDKSKLK	ubiquitination	[5]
21	AEIEKFDKSKLKKTE	acetylation	[1, 3]
21	AEIEKFDKSKLKKTE	ubiquitination	[5]
32	KKTETQEKNPLPSKE	acetylation	[3, 6]
32	KKTETQEKNPLPSKE	succinylation	[6]
38	EKNPLPSKETIEQEK	acetylation	[4, 6]
38	EKNPLPSKETIEQEK	ubiquitination	[5]
45	KETIEQEKQAGES**	ubiquitination	[5]

Reference

 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Aspirin acetylates multiple cellular proteins in HCT-116 colon cancer cells: Identification of novel targets.
 Marimuthu S, Chivukula RS, Alfonso LF, Moridani M, Hagen FK, Bhat GJ.
 Int J Oncol. 2011 Nov;39(5):1273-83. [PMID: 21743961]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. 

Sequence Annotation

 MOD_RES 8 8 N-acetylserine (By similarity).
 MOD_RES 32 32 N6-acetyllysine (By similarity).
 MOD_RES 45 45 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Actin-binding; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 50 AA 

Protein Sequence

Gene Ontology

 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
 GO:0051152; P:positive regulation of smooth muscle cell differentiation; TAS:DFLAT.
 GO:0014911; P:positive regulation of smooth muscle cell migration; TAS:DFLAT.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; TAS:DFLAT.
 GO:0042989; P:sequestering of actin monomers; IEA:InterPro. 

Interpro

 IPR001152; Thymosin_b4.
 IPR016323; Thymosin_b4_chordata. 

Pfam

 PF01290; Thymosin 

SMART

 SM00152; THY 

PROSITE

 PS00500; THYMOSIN_B4 

PRINTS