UniProt Accession

 ARF4_HUMAN; P18085; B2R7J7; P21371 

Genbank Protein ID

 M36341; AF104238; AF104233; AF104234; AF104235; AF104236; AF104237; AF493883; AK313008; BC003364; BC008753; BC016325; BC022866 

Genbank Nucleotide ID

 AAA53081.1; AAD54674.1; AAD54674.1; AAD54674.1; AAD54674.1; AAD54674.1; AAD54674.1; AAM12597.1; BAG35844.1; AAH03364.1; AAH08753.1; AAH16325.1; AAH22866.1 

Protein Name

 ADP-ribosylation factor 4 

Protein Synonyms/Alias

  

Gene Name

 ARF4 

Gene Synonyms/Alias

 ARF2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
15	LFSRLFGKKQMRILM	ubiquitination	[1]
16	FSRLFGKKQMRILMV	ubiquitination	[1]
36	GKTTILYKLKLGEIV	acetylation	[2]
109	EVADELQKMLLVDEL	ubiquitination	[3, 4]
127	VLLLFANKQDLPNAM	ubiquitination	[3, 4]
142	AISEMTDKLGLQSLR	ubiquitination	[1, 3, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. 

Sequence Annotation

 NP_BIND 24 31 GTP.
 NP_BIND 67 71 GTP.
 NP_BIND 126 129 GTP.
 LIPID 2 2 N-myristoyl glycine (Potential).  

Keyword

 3D-structure; Complete proteome; ER-Golgi transport; Golgi apparatus; GTP-binding; Lipoprotein; Myristate; Nucleotide-binding; Polymorphism; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 180 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; TAS:ProtInc.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; IEA:Compara.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0048678; P:response to axon injury; IEA:Compara.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR024156; Small_GTPase_ARF.
 IPR006689; Small_GTPase_ARF/SAR. 

Pfam

 PF00025; Arf 

SMART

 SM00177; ARF 

PROSITE

 PS51417; ARF 

PRINTS

 PR00328; SAR1GTPBP.