CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042027
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Xaa-Pro aminopeptidase 1 
Protein Synonyms/Alias
  
Gene Name
 XPNPEP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
127RYFLQAAKQMDSNWTubiquitination[1]
141TLMKMGLKDTPTQEDubiquitination[2]
173IIPTDYWKKMAKVLRacetylation[3, 4]
173IIPTDYWKKMAKVLRubiquitination[2, 5]
174IPTDYWKKMAKVLRSubiquitination[1, 4]
190GHHLIPVKENLVDKIubiquitination[2]
319KSILSELKALCADLSubiquitination[1]
330ADLSPREKVWVSDKAacetylation[3, 4]
330ADLSPREKVWVSDKAubiquitination[1]
336EKVWVSDKASYAVSEubiquitination[1]
347AVSETIPKDHRCCMPacetylation[4]
365ICIAKAVKNSAESEGubiquitination[1]
408TEISAADKAEEFRRQubiquitination[1]
460GTPTAYEKECFTYVLubiquitination[4]
565VVLVVPVKTKYNFNNubiquitination[1]
567LVVPVKTKYNFNNRGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 642 AA 
Protein Sequence
MAASRKPPRV RVNHQDFQLR NLRIIEPNEV THSGDTGVET DGRMPPKVTS ELLRQLRQAM 60
RNSEYVTEPI QAYIIPSGDA HQSEYIAPCD CRRAFVSGFD GSAGTAIITE EHAAMWTDGR 120
YFLQAAKQMD SNWTLMKMGL KDTPTQEDWL VSVLPEGSRV GVDPLIIPTD YWKKMAKVLR 180
SAGHHLIPVK ENLVDKIWTD RPERPCKPLL TLGLDYTGIS WKDKVADLRL KMAERNVMWF 240
VVTALDEIAW LFNLRGSDVE HNPVFFSYAI IGLETIMLFI DGDRIDAPSV KEHLLLDLGL 300
EAEYRIQVHP YKSILSELKA LCADLSPREK VWVSDKASYA VSETIPKDHR CCMPYTPICI 360
AKAVKNSAES EGMRRAHIKD AVALCELFNW LEKEVPKGGV TEISAADKAE EFRRQQADFV 420
DLSFPTISST GPNGAIIHYA DGTTDVTRTM HFGTPTAYEK ECFTYVLKGH IAVSAAVFPT 480
GTKGHLLDSF ARSALWDSGL DYLHGTGHGV GSFLNVHEGP CGISYKTFSD EPLEAGMIVT 540
DEPGYYEDGA FGIRIENVVL VVPVKTKYNF NNRGSLTFEP LTLVPIQTKM IDVDSLTDKE 600
CDWLNNYHLT CRDVIGKELQ KQGRQEALEW LIRETQPISK QH 642 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009987; P:cellular process; IEA:InterPro. 
Interpro
 IPR000587; Creatinase.
 IPR000994; Pept_M24_structural-domain.
 IPR001131; Peptidase_M24B_aminopep-P_CS. 
Pfam
 PF01321; Creatinase_N
 PF00557; Peptidase_M24 
SMART
  
PROSITE
 PS00491; PROLINE_PEPTIDASE 
PRINTS