| Tag | Content |
|---|
| CPLM ID | CPLM-006153 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Translation initiation factor eIF-2B subunit beta |
Protein Synonyms/Alias | GCD complex subunit GCD7; Guanine nucleotide exchange factor subunit GCD7; eIF-2B GDP-GTP exchange factor subunit beta |
Gene Name | GCD7 |
Gene Synonyms/Alias | TIF222; YLR291C; L8003.17 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 311 | LYPFDVEKFVEFGGS | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Acts as a regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site. |
Sequence Annotation | |
Keyword | Complete proteome; Initiation factor; Protein biosynthesis; Reference proteome; Translation regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 381 AA |
Protein Sequence | MSSQAFTSVH PNAATSDVNV TIDTFVAKLK RRQVQGSYAI ALETLQLLMR FISAARWNHV 60
NDLIEQIRDL GNSLEKAHPT AFSCGNVIRR ILAVLRDEVE EDTMSTTVTS TSVAEPLISS 120
MFNLLQKPEQ PHQNRKNSSG SSSMKTKTDY RQVAIQGIKD LIDEIKNIDE GIQQIAIDLI 180
HDHEILLTPT PDSKTVLKFL ITARERSNRT FTVLVTEGFP NNTKNAHEFA KKLAQHNIET 240
LVVPDSAVFA LMSRVGKVII GTKAVFVNGG TISSNSGVSS VCECAREFRT PVFAVAGLYK 300
LSPLYPFDVE KFVEFGGSQR ILPRMDPRKR LDTVNQITDY VPPENIDIYI TNVGGFNPSF 360
IYRIAWDNYK QIDVHLDKNK A 381 |
Gene Ontology | GO:0005851; C:eukaryotic translation initiation factor 2B complex; IDA:SGD. GO:0032045; C:guanyl-nucleotide exchange factor complex; IDA:SGD. GO:0005739; C:mitochondrion; IDA:SGD. GO:0030234; F:enzyme regulator activity; IMP:SGD. GO:0003743; F:translation initiation factor activity; IGI:SGD. GO:0006446; P:regulation of translational initiation; IDA:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |