CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023561
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 EH domain-containing protein 1 
Protein Synonyms/Alias
 PAST homolog 1; mPAST1 
Gene Name
 Ehd1 
Gene Synonyms/Alias
 Past1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
7*MFSWVSKDARRKKEacetylation[1]
12VSKDARRKKEPELFQubiquitination[2]
32LRQLYAQKLLPLEEHubiquitination[2]
162PGILSGEKQRISRGYubiquitination[2]
269LLIPDNRKLFEAEEQubiquitination[2]
341NLGEIYQKIEREHQIubiquitination[2]
358GDFPSLRKMQELLQTubiquitination[2]
370LQTQDFSKFQALKPKubiquitination[2]
492WKLADVDKDGLLDDEacetylation[1]
509ALANHLIKVKLEGHEubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. 
Sequence Annotation
 DOMAIN 444 532 EH.
 DOMAIN 476 511 EF-hand.
 NP_BIND 65 72 ATP (By similarity).
 BINDING 220 220 ATP (By similarity).
 BINDING 258 258 ATP (By similarity).
 MOD_RES 456 456 Phosphoserine (By similarity).  
Keyword
 ATP-binding; Calcium; Cell membrane; Coiled coil; Complete proteome; Endosome; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 534 AA 
Protein Sequence
MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL EDADFDNKPM 60
VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV MHGPTEGVVP GNALVVDPRR 120
PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS IIDTPGILSG EKQRISRGYD FAAVLEWFAE 180
RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL 240
GKIINTPEVV RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR 300
ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISS GDFPSLRKMQ 360
ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE ESLMPSQAVK GGAFDGTMNG 420
PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE IFYTLSPVNG KITGANAKKE MVKSKLPNTV 480
LGKIWKLADV DKDGLLDDEE FALANHLIKV KLEGHELPAD LPPHLIPPSK RRHE 534 
Gene Ontology
 GO:0031901; C:early endosome membrane; ISS:UniProtKB.
 GO:0030139; C:endocytic vesicle; IDA:MGI.
 GO:0005811; C:lipid particle; IDA:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0031095; C:platelet dense tubular network membrane; IDA:BHF-UCL.
 GO:0055038; C:recycling endosome membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0042632; P:cholesterol homeostasis; IMP:BHF-UCL.
 GO:0032456; P:endocytic recycling; ISS:UniProtKB.
 GO:0006897; P:endocytosis; IDA:MGI.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0006886; P:intracellular protein transport; ISS:UniProtKB.
 GO:0034383; P:low-density lipoprotein particle clearance; IMP:BHF-UCL.
 GO:0010886; P:positive regulation of cholesterol storage; IMP:BHF-UCL.
 GO:2001137; P:positive regulation of endocytic recycling; IMP:UniProtKB.
 GO:1901741; P:positive regulation of myoblast fusion; IMP:UniProtKB.
 GO:0051260; P:protein homooligomerization; IEA:Compara. 
Interpro
 IPR001401; Dynamin_GTPase.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00350; Dynamin_N 
SMART
 SM00027; EH 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH 
PRINTS