CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024351
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase UBR4 
Protein Synonyms/Alias
 N-recognin-4; Zinc finger UBR1-type protein 1; p600 
Gene Name
 Ubr4 
Gene Synonyms/Alias
 Kiaa0462; Zubr1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
187VSPELRQKEVQMNFLubiquitination[1]
634NKQASGEKGNILASRubiquitination[1]
1149AAETDPHKSSEITKNubiquitination[1]
1573CKKYLSQKNVVEKLNubiquitination[1]
3813NSFDELSKIIQKVFAacetylation[2]
3838QQREAATKSSRTSVQacetylation[3]
4019MCLRILQKLIKPPAPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 
Functional Description
 E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. 
Sequence Annotation
 ZN_FING 1655 1728 UBR-type.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 1083 1083 N6-acetyllysine (By similarity).
 MOD_RES 1401 1401 Phosphoserine (By similarity).
 MOD_RES 2712 2712 Phosphothreonine.
 MOD_RES 2716 2716 Phosphoserine.
 MOD_RES 2719 2719 Phosphoserine.
 MOD_RES 2721 2721 Phosphothreonine.  
Keyword
 Acetylation; Alternative splicing; Calmodulin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Ligase; Membrane; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 5180 AA 
Protein Sequence
MATSGGEEAA AAAPAPGAPA TGQDTTPGWE VAVRPLLSAS YSAFEMKELP QLVASVIESE 60
SEILHHEKQY EPFYSSFVAL STHYITTVCS LIPRNQLQSV AAACKVLIEF SLLRLENPDE 120
ACAVSQKHLI LLIKGLCTGC SRLDRTEIIT FTAMMKSAKL PQTVKTLSDV EDQKELASPV 180
SPELRQKEVQ MNFLNQLTSV FNPRTVPSPP ISPQALVEGE NDEQSSPDQV SAAKTKSVFI 240
AQNVASLQEL GGSEKLLRVC LNLPYFLRYI NRFQDAVVAN SFFIMPATVA DATAVRNGFH 300
SLVIDVTMAL DTLSLPVLEP LNPSRLQDVT VLSLSCLYAG VSVATCMAIL HVGSAQQVRT 360
GSTSSKEDDY ESDAATIVQK CLEIYDMIGQ AISSSRRAGG EHFQNFQLLG AWCLLNSLFL 420
ILNLSPTALA DKGKEKDPLA ALRVRDILSR TKEGVGSPKL GPGKGHQGFG VLSVILANHA 480
IKLLASLFQD LQVEALHKGW ETDGPPAVLS IMAQSTSTQR IQRLIDSVPL TNLLLTLLST 540
SYRKACVLQR QRKGSMSSDA SASTDSNTYY EDDFSSTEED SSQDDDSEPI LGQWFEETIS 600
PSKEKAAPPP PPPPPPLESS PRVKSPNKQA SGEKGNILAS RKDPELFSGL ASNILNFITT 660
SMLNSRNSFI RSYLSASLSE HHMATLASII KEVDKDGLKG SSDEDFAAAL YHFNHSLVTS 720
DLQSPNLQNT LLQQLGVAPF SEGPWPLYIH PQGLSVLSRL LLIWQHKAGA QGDPDVPECL 780
KVWDRFLTTM KQNALQGVVP SETEDLNVEH LQLLLLIFHS FSEKGRRAIL TMLVQSIQEL 840
SVNMEVQMRT APLILARLLL IFDYLLHQYS KAPVYLFEQV QHNLLSPPFG WASGSQDSSS 900
RRANTPLYHG FKEVEENWSK HFSSDAAPQP RFYCVLSTEA SEEDLNRLDS EACEVLFSKP 960
VKYDELYSSL TTLLAAGSQL DTTRRKEKKN VTALEACALQ YYFLILWRIL GILPPSKTYM 1020
NQLAMNSPEM SECDILHTLR WSSRLRISSY VSWIKDHLIK QGMKPEHAGS LIELAASKCS 1080
SVKYDVEIVE EYFARQISSF CSIDCTAVLQ LHEIPSLQSI YTLDAAVSKV QVSLDEHFSK 1140
MAAETDPHKS SEITKNLLPA TLQLIDTYAS FTRAYLLQNL NEEGSTEKPS QEKLHGFAAV 1200
LAIGSSRCKA NTLGPTLVQN LPSSVQSVCE SWNNINTNEF PNIGSWRNAF ANDTIPSESY 1260
ISAVQAAHLG TLCGQSLPLA ASLKHTLLSL VRLTGDLIVW SDEMNPAQVI RTLLPLLLES 1320
STESAAEISS NSLERILGPA ESDEFLARVY EKLITGCYNI LANHADPNSG LDESILEECL 1380
QYLEKQLESS QARKAMEEFF SDGGELVQIM MATANEDLSA KFCNRVLKFF TKLFQLTEKS 1440
PNPSLLHLCG SLAQLACVEP VRLQAWLTRM TTSPPKDSDQ LEVIQENRQL LQLLTTYIVR 1500
ENSQVGEGVC AVLLGTLTPM ATDMLANGDG TGFPELMVVM ATLASAGQGA GHLQLHNAAV 1560
DWLGRCKKYL SQKNVVEKLN ANVMHGKHVM VLECTCHIMS YLADVTNALS QSNGQGPSHL 1620
SVDGEERAIE VDSDWVEELA VEEEDSQAED SDEDSLCNKL CTFTITQKEF MNQHWYHCHT 1680
CKMVDGVGVC TVCAKVCHKD HEISYAKYGS FFCDCGAKED GSCLALVKRT PSSGMSSTMK 1740
ESAFQSEPRV SESLVRHAST SPADKAKVTI SDGKVTDEEK PKKSSLCRTV EGCREELQNQ 1800
ANFSFAPLVL DMLSFLMDAI QTNFQQASAV GSSSRAQQAL SELHTVDKGV EMTDQLMVPT 1860
LGSQEGAFEN VRMNYSGDQG QTIRQLISAH VLRRVAMCVL SSPHGRRQHL AVSHEKGKIT 1920
VLQLSALLKQ ADSSKRKLTL TRLASAPVPF TVLSLTGNPC KEDYLAVCGL KDCHVLTFSS 1980
SGSVSDHLVL HPQLATGNFI IKAVWLPGSQ TELAIVTADF VKIYDLSIDA LSPTFYFLLP 2040
SSKIRDVTFL FNEEGKNIIV IMSSAGYMYT QLMEEASSAQ QGPFYVTNVL EINHEDLKDS 2100
NSQVAGGGVS VYYSHVLQML FFSYSQGRSF AATVSRSTLE VLQLFPINIK SSNGGSKTSP 2160
ALCQWSEVMN HPGLVCCVQQ TTGVPLVVMV KPGTFLIQEI KTLPAKAKIQ DMVAIRHTAC 2220
NEQQRTTMIL LCEDGSLRIY MANVENTSYW LQPSLQPSSV ISIMKPVRKR KTATITARTS 2280
SQVTFPIDFF EHNQQLTDVE FGGNDLLQVY NAQQIKHRLN STGMYVANTK PGGFTIEISN 2340
NSSTMVMTGM RIQIGTQAIE RAPSYIEIFG RTMQLNLSRS RWFDFPFTRE EALQADRKLS 2400
LFIGASVDPA GVTMIDAVKI YGKTKEQFGW PDEPPEDFPS ASVSNICPPN LNQSNGTGES 2460
DSAAPATTSG TVLERLVVSS LEALESCFAV GPIIEKERNK HAAQELATLL LSLPAPASVQ 2520
QQSKSLLASL HSSRSAYHSH KDQALLSKAV QCLNTSSKEG KDLDPEVFQR LVITARSIAV 2580
TRPNNLVHFT ESKLPQMETE GADEGKEPQK QEGDGCSFIT QLVNHFWKLH ASKPKNAFLA 2640
PACLPGLTHI EATVNALVDI IHGYCTCELD CINTASKIYM QMLLCPDPAV SFSCKQALIR 2700
VLRPRNKRRH VTLPSSPRSN TPMGDKDDDD DDDADEKMQS SGIPDGGHIR QESQEQSEVD 2760
HGDFEMVSES MVLETAENVN NGNPSPLEAL LAGAEGFPPM LDIPPDADDE TMVELAIALS 2820
LQQDQQGSSS SALGLQSLGL SGQAPSSSSL DAGTLSDTTA SAPASDDEGS TAATDGSTLR 2880
TSPADHGGSV GSESGGSAVD SVAGEHSVSG RSSAYGDATA EGHPAGPGSV SSSTGAISTA 2940
TGHQEGDGSE GEGEGEAEGD VHTSNRLHMV RLMLLERLLQ TLPQLRNVGG VRAIPYMQVI 3000
LMLTTDLDGE DEKDKGALDN LLAQLIAELG MDKKDVSKKN ERSALNEVHL VVMRLLSVFM 3060
SRTKSGSKSS ICESSSLISS ATAAALLSSG AVDYCLHVLK SLLEYWKSQQ SDEEPVAASQ 3120
LLKPHTTSSP PDMSPFFLRQ YVKGHAADVF EAYTQLLTEM VLRLPYQIKK IADTSSRIPP 3180
PVFDHSWFYF LSEYLMIQQT PFVRRQVRKL LLFICGSKEK YRQLRDLHTL DSHVRGIKKL 3240
LEEQGIFLRA SVVTASSGSA LQYDTLISLM EHLKACAEIA AQRTINWQKF CIKDDSVLYF 3300
LLQVSFLVDE GVSPVLLQLL SCALCGSKVL AALAASTGSS SVASSSAPPA ASSGQATTQS 3360
KSSTKKSKKE EKEKEKEGES SGSQEDQLCT ALVNQLNRFA DKETLIQFLR CFLLESNSSS 3420
VRWQAHCLTL HIYRNSNKAQ QELLLDLMWS IWPELPAYGR KAAQFVDLLG YFSLKTAQTE 3480
KKLKEYSQKA VEILRTQNHI LTNHPNSNIY NTLSGLVEFD GYYLESDPCL VCNNPEVPFC 3540
YIKLSSIKVD TRYTTTQQVV KLIGSHTISK VTVKIGDLKR TKMVRTINLY YNNRTVQAIV 3600
ELKNKPARWH KAKKVQLTPG QTEVKIDLPL PIVASNLMIE FADFYENYQA STETLQCPRC 3660
SASVPANPGV CGNCGENVYQ CHKCRSINYD EKDPFLCNAC GFCKYARFDF MLYAKPCCAV 3720
DPIENEEDRK KAVSNINTLL DKADRVYHQL MGHRPQLENL LCKVNEAAPE KPQEDSGTAG 3780
GISSTSASVN RYILQLAQEY CGDCKNSFDE LSKIIQKVFA SRKELLEYDL QQREAATKSS 3840
RTSVQPTFTA SQYRALSVLG CGHTSSTKCY GCASAVTEHC ITLLRALATN PALRHILVSQ 3900
GLIRELFDYN LRRGAAAIRE EVRQLMCLLT RDNPEATQQM NDLIIGKVST ALKGHWANPD 3960
LASSLQYEML LLTDSISKED SCWELRLRCA LSLFLMAVNI KTPVVVENIT LMCLRILQKL 4020
IKPPAPTSKK NKDVPVEALT TVKPYCNEIH AQAQLWLKRD PKASYEAWKK CLPIRGVDGN 4080
GKSPSKSELH RLYLTEKYVW RWKQFLSRRG KRTTPLDLKL GHNNWLRQVL FTPATQAARQ 4140
AACTIVEALA TVPSRKQQVL DLLTSYLDEL SVAGECAAEY LALYQKLIAS CHWKVYLAAR 4200
GVLPYVGNLI TKEIARLLAL EEATLSTDLQ QGYALKSLTG LLSSFVEVES IKRHFKSRLV 4260
GTVLNGYLCL RKLVLQRTKL IDETQDMLLE MLEDMTTGTE SETKAFMAVC IETAKRYNLD 4320
DYRTPVFIFE RLCSIIYPEE NEVTEFFVTL EKDPQQEDFL QGRMPGNPYS SNEPGIGPLM 4380
RDIKNKICQD CDLVALLEDD SGMELLVNNK IISLDLPVAE VYKKVWCATN EGEPMRIVYR 4440
MRGLLGDATE EFIESLDSTT DEEEDEEEVY RMAGVMAQCG GLQCMLNRLA GVKDFKQGRH 4500
LLTVLLKLFS YCVKVKVNRQ QLVKLETNTL NVMLGTLNLA LVAEQESKDS GGAAVAEQVL 4560
SIMEIILDES NAEPLSEDKG NLLLTGDKDQ LVMLLDQINS TFVRSNPSVL QGLLRIIPYL 4620
SFGEVEKMQI LVERFKPYCS FEKYDEDHSG DDKVFLDCFC KIAAGIKNNS NGHQLKDLIL 4680
QKGITQNALD YMKKHIPSAK NLDADIWKKF LSRPALPFIL RLLRGLAMQH PATQVLIGTD 4740
SITSLHKLEQ VSSDEGIGTL AENLLEALRE HPDVNKKIDA ARRETRAEKK RMAMAMRQKA 4800
LGTLGMTTNE KGQVVTKTAL LKQMEELIEE PGLTCCICRE GYKFQPTKVL GIYTFTKRVA 4860
LEEMENKPRK QQGYSTVSHF NIVHYDCHLA AVRLARGREE WESAALQNAN TKCNGLLPVW 4920
GPHVPESAFA TCLARHNTYL QECTGQREPT YQLNIHDIKL LFLRFAMEQS FSADTGGGGR 4980
ESNIHLIPYI IHTVLYVLNT TRATSREEKN LQGFLEQPKE KWTESAFDVD GPHYFTILAL 5040
HVLPPEQWKA IRVEILRRLL VASHARAVAP GGATRLTDKA VKDYSAYRSS LLFWALVDLI 5100
YNMFKKVPTS NTEGGWSCSL AEYIRHNDMP IYEAADKALK TFQEEFMPVE TFSEFLDAAG 5160
LLSEITDPES FLKDLLNSVP 5180 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR025704; E3_Ub_ligase_UBR4.
 IPR003126; Znf_N-recognin. 
Pfam
 PF13764; E3_UbLigase_R4
 PF02207; zf-UBR 
SMART
  
PROSITE
 PS51157; ZF_UBR 
PRINTS