UniProt Accession

 SAR1_YEAST; P20606; D6W3F2 

Genbank Protein ID

 X51667; Z73574; BK006949 

Genbank Nucleotide ID

 CAA35978.1; CAA97933.1; DAA11218.1 

Protein Name

 Small COPII coat GTPase SAR1 

Protein Synonyms/Alias

 GTP-binding protein SAR1; Secretion-associated RAS-related protein 1 

Gene Name

 SAR1 

Gene Synonyms/Alias

 YPL218W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
44	TTLLHMLKNDRLATL	acetylation	[1]
68	ELAIGNIKFTTFDLG	ubiquitination	[2]
133	PFVILGNKIDAPNAV	ubiquitination	[2, 3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Small GTPase component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. SAR1 controls the coat assembly in a stepwise manner. Activated SAR1-GTP by SEC12 binds to membranes first and recruits the SEC23/24 complex. These SEC23/24-SAR1 prebudding intermediates are then collected by the SEC13/31 complex as subunits polymerize to form coated transport vesicles. Conversion to SAR1-GDP triggers coat release and recycles COPII subunits. 

Sequence Annotation

 NP_BIND 30 37 GTP.
 NP_BIND 73 76 GTP.
 NP_BIND 132 135 GTP.
 MOD_RES 155 155 Phosphothreonine.
 MOD_RES 157 157 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasmic vesicle; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; GTP-binding; Hydrolase; Membrane; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 190 AA 

Protein Sequence

Gene Ontology

 GO:0030127; C:COPII vesicle coat; IDA:SGD.
 GO:0070971; C:endoplasmic reticulum exit site; IDA:SGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:SGD.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0003400; P:regulation of COPII vesicle coating; IDA:SGD.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR006689; Small_GTPase_ARF/SAR.
 IPR006687; Small_GTPase_SAR1. 

Pfam

 PF00025; Arf 

SMART

 SM00178; SAR 

PROSITE

 PS51422; SAR1 

PRINTS

 PR00328; SAR1GTPBP.