UniProt Accession

 NFS1_HUMAN; Q9Y697; B3KMA5; B4DXK9; E1P5R8; F5GYK5; Q6P0L8; Q9NTZ5; Q9Y481 

Genbank Protein ID

 AF097025; AK001470; AK302023; AL109827; AL357374; AL109827; AL357374; CH471077; CH471077; BC065560; AJ010952 

Genbank Nucleotide ID

 AAD09187.2; BAG50917.1; BAG63421.1; CAB87612.2; CAB87612.2; CAI20116.1; CAI20116.1; EAW76170.1; EAW76172.1; AAH65560.1; CAA09424.1 

Protein Name

 Cysteine desulfurase, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 NFS1 

Gene Synonyms/Alias

 NIFS; HUSSY-08 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
157	ITTQTEHKCVLDSCR	ubiquitination	[1]
180	VTYLPVQKSGIIDLK	ubiquitination	[1, 2]
212	VNNEIGVKQPIAEIG	ubiquitination	[2]
239	DAAQAVGKIPLDVND	ubiquitination	[1]
248	PLDVNDMKIDLMSIS	ubiquitination	[1]
263	GHKIYGPKGVGAIYI	ubiquitination	[1]
335	RLIQNIMKSLPDVVM	ubiquitination	[1]
453	GIDLKSIKWTQH***	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron- sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor. 

Sequence Annotation

 ACT_SITE 381 381 By similarity.
 MOD_RES 258 258 N6-(pyridoxal phosphate)lysine (By  

Keyword

 Alternative initiation; Alternative splicing; Complete proteome; Cytoplasm; Mitochondrion; Molybdenum cofactor biosynthesis; Nucleus; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 457 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:HGNC.
 GO:0005759; C:mitochondrial matrix; IDA:HGNC.
 GO:0005634; C:nucleus; IDA:HGNC.
 GO:0031071; F:cysteine desulfurase activity; IDA:HGNC.
 GO:0042803; F:protein homodimerization activity; IDA:HGNC.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0006534; P:cysteine metabolic process; IEA:InterPro.
 GO:0018283; P:iron incorporation into metallo-sulfur cluster; IDA:HGNC.
 GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; IEA:UniProtKB-KW.
 GO:0032324; P:molybdopterin cofactor biosynthetic process; TAS:Reactome.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0000096; P:sulfur amino acid metabolic process; TAS:ProtInc.
 GO:0006767; P:water-soluble vitamin metabolic process; TAS:Reactome. 

Interpro

 IPR000192; Aminotrans_V/Cys_dSase.
 IPR020578; Aminotrans_V_PyrdxlP_BS.
 IPR010240; Cys_deSase.
 IPR016454; Cysteine_dSase_NifS.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 

Pfam

 PF00266; Aminotran_5 

SMART

  

PROSITE

 PS00595; AA_TRANSFER_CLASS_5 

PRINTS