UniProt Accession

 BCA1_YEAST; P38891; D3DLF7 

Genbank Protein ID

 X78961; U00029; AY558111; BK006934 

Genbank Nucleotide ID

 CAA55556.1; AAB69733.1; AAS56437.1; DAA06901.1 

Protein Name

 Branched-chain-amino-acid aminotransferase, mitochondrial 

Protein Synonyms/Alias

 BCAT; Protein ECA39; Protein TWT1 

Gene Name

 BAT1 

Gene Synonyms/Alias

 ECA39; TWT1; YHR208W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
113	ITMFRPDKNMARMNK	acetylation	[1]
141	ELIKLTGKLIEQDKH	acetylation	[1]
147	GKLIEQDKHLVPQGN	acetylation	[1]
197	PYYKTGFKAVRLEAT	acetylation	[1]
218	WPGGVGDKKLGANYA	acetylation	[1]
299	VLTLARDKLDPQEWD	acetylation	[1]
322	TEVATRAKQGELLEA	ubiquitination	[2]
387	VNYGNWSKTVADLN*	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Involved in the biosynthesis of the branched chain amino acids leucine, isoleucine, and valine. Catalyzes the formation of methionine from 2-keto-4-methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using branched chain amino acids (leucine, isoleucine, and valine) as the amino donors. Appears to be involved in the regulation of the transition from G1 to S phase in the cell cycle. High copy suppressor of a temperature-sensitive mutation in the ABC transporter, ATM1. 

Sequence Annotation

 MOD_RES 219 219 N6-(pyridoxal phosphate)lysine (By
 MOD_RES 315 315 Phosphothreonine.  

Keyword

 Amino-acid biosynthesis; Aminotransferase; Branched-chain amino acid biosynthesis; Complete proteome; Mitochondrion; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 393 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IDA:SGD.
 GO:0004084; F:branched-chain-amino-acid transaminase activity; IDA:SGD.
 GO:0052656; F:L-isoleucine transaminase activity; IEA:EC.
 GO:0052654; F:L-leucine transaminase activity; IEA:EC.
 GO:0052655; F:L-valine transaminase activity; IEA:EC.
 GO:0009082; P:branched-chain amino acid biosynthetic process; IMP:SGD.
 GO:0009083; P:branched-chain amino acid catabolic process; IMP:SGD.
 GO:0034644; P:cellular response to UV; IMP:MGI.
 GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; IEA:UniProtKB-UniPathway.
 GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0031571; P:mitotic G1 DNA damage checkpoint; IMP:MGI.
 GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR001544; Aminotrans_IV.
 IPR018300; Aminotrans_IV_CS.
 IPR005786; B_amino_transII. 

Pfam

 PF01063; Aminotran_4 

SMART

  

PROSITE

 PS00770; AA_TRANSFER_CLASS_4 

PRINTS