CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024040
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 SUMO-protein ligase PIAS3 
Protein Synonyms/Alias
 Protein inhibitor of activated STAT protein 3 
Gene Name
 PIAS3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MAELGELKHMVMSFRubiquitination[1]
56CAPSVQMKIKELYRRubiquitination[1]
58PSVQMKIKELYRRRFubiquitination[1]
183REVLPGAKCDYTIQVubiquitination[1]
236TKNGAEPKRPSRPINubiquitination[1]
288TAGTLLQKLRAKGIRubiquitination[1]
305DHSRALIKEKLTADPubiquitination[1]
307SRALIKEKLTADPDSubiquitination[1, 2]
331SLMCPLGKMRLTVPCubiquitination[1]
372TCPVCDKKAPYESLIubiquitination[1]
442GGDPSENKKKVEVIDubiquitination[1]
443GDPSENKKKVEVIDLubiquitination[1]
482IPALPGSKGVLTSGHubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. 
Sequence Annotation
 DOMAIN 11 45 SAP.
 DOMAIN 115 280 PINIT.
 ZN_FING 303 380 SP-RING-type.
 REGION 450 460 SUMO1-binding (By similarity).
 MOTIF 19 23 LXXLL motif.  
Keyword
 Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 628 AA 
Protein Sequence
MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA PSVQMKIKEL 60
YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL LAPGTLLGPK REVDMHPPLP 120
QPVHPDVTMK PLPFYEVYGE LIRPTTLAST SSQRFEEAHF TFALTPQQVQ QILTSREVLP 180
GAKCDYTIQV QLRFCLCETS CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR 240
PINITPLARL SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS 300
RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD AALYLQMNEK 360
KPTWTCPVCD KKAPYESLII DGLFMEILSS CSDCDEIQFM EDGSWCPMKP KKEASEVCPP 420
PGYGLDGLQY SPVQGGDPSE NKKKVEVIDL TIESSSDEED LPPTKKHCSV TSAAIPALPG 480
SKGVLTSGHQ PSSVLRSPAM GTLGGDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ 540
HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP GRVSSIVAPG 600
GALREGHGGP LPSGPSLTGC RSDIISLD 628 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0045202; C:synapse; IEA:Compara.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0015459; F:potassium channel regulator activity; IEA:Compara.
 GO:0019789; F:SUMO ligase activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0045838; P:positive regulation of membrane potential; IEA:Compara.
 GO:0033235; P:positive regulation of protein sumoylation; IDA:UniProtKB.
 GO:0016925; P:protein sumoylation; ISS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0009725; P:response to hormone stimulus; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR027226; PIAS3.
 IPR023321; PINIT.
 IPR003034; SAP_dom.
 IPR004181; Znf_MIZ. 
Pfam
 PF14324; PINIT
 PF02891; zf-MIZ 
SMART
 SM00513; SAP 
PROSITE
 PS51466; PINIT
 PS50800; SAP
 PS51044; ZF_SP_RING 
PRINTS