CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024547
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription activator BRG1 
Protein Synonyms/Alias
 ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 
Gene Name
 SMARCA4 
Gene Synonyms/Alias
 BAF190A; BRG1; SNF2B; SNF2L4 
Created Date
 July 27, 2013 
Organism
 Bos taurus (Bovine) 
NCBI Taxa ID
 9913 
Lysine Modification
Position
Peptide
Type
References
349SRITPIQKPRGLDPVubiquitination[1]
391LAGDLRTKATIELKAubiquitination[1]
397TKATIELKALRLLNFubiquitination[1]
429LETALNAKAYKRSKRubiquitination[1]
447REARITEKLEKQQKIubiquitination[1]
450RITEKLEKQQKIEQEubiquitination[1]
488YHRSVTGKIQKLTKAubiquitination[1]
703RHIIENAKQDVDDEYubiquitination[1]
989EKVEYVIKCDMSALQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-fOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self- renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (By similarity). 
Sequence Annotation
 DOMAIN 171 206 QLQ.
 DOMAIN 452 524 HSA.
 DOMAIN 758 923 Helicase ATP-binding.
 DOMAIN 1076 1238 Helicase C-terminal.
 DOMAIN 1436 1506 Bromo.
 NP_BIND 771 778 ATP (Potential).
 REGION 1 274 Necessary for interaction with
 MOTIF 873 876 DEGH box (By similarity).
 MOD_RES 11 11 Phosphothreonine (By similarity).
 MOD_RES 188 188 N6-acetyllysine (By similarity).
 MOD_RES 345 345 Phosphothreonine (By similarity).
 MOD_RES 601 601 Phosphothreonine (By similarity).
 MOD_RES 602 602 Phosphoserine (By similarity).
 MOD_RES 605 605 Phosphoserine (By similarity).
 MOD_RES 687 687 Phosphoserine (By similarity).
 MOD_RES 691 691 Phosphoserine (By similarity).
 MOD_RES 1341 1341 Phosphoserine (By similarity).
 MOD_RES 1411 1411 Phosphoserine (By similarity).
 MOD_RES 1529 1529 Phosphoserine (By similarity).
 MOD_RES 1534 1534 Phosphoserine (By similarity).
 MOD_RES 1545 1545 Phosphoserine (By similarity).
 MOD_RES 1586 1586 Phosphoserine (By similarity).
 MOD_RES 1590 1590 Phosphoserine (By similarity).  
Keyword
 Acetylation; Activator; ATP-binding; Bromodomain; Chromatin regulator; Complete proteome; Helicase; Hydrolase; Neurogenesis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1606 AA 
Protein Sequence
MSTPDPALGG TPRPGPSPGP GPSPGAMLGP SPGPSPGSAH SIMGPSPGPP SAGHPIPTQG 60
PGGYPQDNMH QMHKPMESMH EKGMSDDPRY TQMKGMGMRS GGHAGMGPPP SPMDQHSQGY 120
PSPLGGSEHA SSPVPASGPS SGPQMSSGPG GAPLDGADPQ ALGQQNRGPT PFNQNQLHQL 180
RAQIMAYKML ARGQPLPDHL QMAVQGKRPM PGMQPQMPAL PPPSVSATGP GPSPGPAPPN 240
YSRPHGMGGP NMPPPGPSGV PPGMPGQPPG GPPKPWPEGP MANAAAPTST PQKLIPPQPT 300
GRPSPAPPAV PPAASPVMPP QTQSPGQPAQ PAPMVPLHQK QSRITPIQKP RGLDPVEILQ 360
EREYRLQARI AHRIQELENL PGSLAGDLRT KATIELKALR LLNFQRQLRQ EVVVCMRRDT 420
ALETALNAKA YKRSKRQSLR EARITEKLEK QQKIEQERKR RQKHQEYLNS ILQHAKDFKE 480
YHRSVTGKIQ KLTKAVATYH ANTEREQKKE NERIEKERMR RLMAEDEEGY RKLIDQKKDK 540
RLAYLLQQTD EYVANLTELV RQHKAAQVAK EKKKKKKKKK AENAEGQTPA IGPDGEPLDE 600
TSQMSDLPVK VIHVESGKIL TGTDAPKAGQ LEAWLEMNPG YEVAPRSDSE ESGSEEEEEE 660
EEEEQPQPAQ PPTLPVEEKK KIPDPDSDDV SEVDARHIIE NAKQDVDDEY GVSQALARGL 720
QSYYAVAHAV TERVDKQSAL MVNGVLKQYQ IKGLEWLVSL YNNNLNGILA DEMGLGKTIQ 780
TIALITYLME HKRINGPFLI IVPLSTLSNW AYEFDKWAPS VVKVSYKGSP AARRAFVPQL 840
RSGKFNVLLT TYEYIIKDKH ILAKIRWKYM IVDEGHRMKN HHCKLTQVLN THYVAPRRLL 900
LTGTPLQNKL PELWALLNFL LPTIFKSCST FEQWFNAPFA MTGEKVDLNE EETILIIRRL 960
HKVLRPFLLR RLKKEVEAQL PEKVEYVIKC DMSALQRVLY RHMQAKGVLL TDGSEKDKKG 1020
KGGTKTLMNT IMQLRKICNH PYMFQHIEES FSEHLGFTGG IVQGLDLYRA SGKFELLDRI 1080
LPKLRATNHK VLLFCQMTSL MTIMEDYFAY RGFKYLRLDG TTKAEDRGML LKTFNEPGSE 1140
YFIFLLSTRA GGLGLNLQSA DTVIIFDSDW NPHQDLQAQD RAHRIGQQNE VRVLRLCTVN 1200
SVEEKILAAA KYKLNVDQKV IQAGMFDQKS SSHERRAFLQ AILEHEEQDE EEDEVPDDET 1260
VNQMIARHEE EFDLFMRMDL DRRREEARNP KRKPRLMEED ELPSWIIKDD AEVERLTCEE 1320
EEEKMFGRGS RHRKEVDYSD SLTEKQWLKA IEEGTLEEIE EEVRQKKSSR KRKRDSDAGP 1380
STPTTSTRSR DKDDESKKQK KRGRPPAEKL SPNPPNLTKK MKKIVDAVIK YKDSSSGRQL 1440
SEVFIQLPSR KELPEYYELI RKPVDFKKIK ERIRNHKYRS LNDLEKDVML LCQNAQTFNL 1500
EGSLIYEDSI VLQSVFTSVR QKIEKEDDSE GEESEEEEEG EEEGSESESR SVKVKIKLGR 1560
KEKAQDRLKG GRRRPSRGSR AKPVVSDDDS EEEQEEDRSG SGSEED 1606 
Gene Ontology
 GO:0000792; C:heterochromatin; IEA:Compara.
 GO:0071565; C:nBAF complex; ISS:UniProtKB.
 GO:0071564; C:npBAF complex; ISS:UniProtKB.
 GO:0005719; C:nuclear euchromatin; IEA:Compara.
 GO:0005726; C:perichromatin fibrils; IEA:Compara.
 GO:0016514; C:SWI/SNF complex; IEA:Compara.
 GO:0071778; C:WINAC complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0008094; F:DNA-dependent ATPase activity; IEA:Compara.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IEA:Compara.
 GO:0001105; F:RNA polymerase II transcription coactivator activity; IEA:Compara.
 GO:0003714; F:transcription corepressor activity; IEA:Compara.
 GO:0035887; P:aortic smooth muscle cell differentiation; IEA:Compara.
 GO:0001832; P:blastocyst growth; IEA:Compara.
 GO:0001835; P:blastocyst hatching; IEA:Compara.
 GO:0000902; P:cell morphogenesis; IEA:Compara.
 GO:0006338; P:chromatin remodeling; IEA:Compara.
 GO:0060318; P:definitive erythrocyte differentiation; IEA:Compara.
 GO:0010424; P:DNA methylation on cytosine within a CG sequence; IEA:Compara.
 GO:0035116; P:embryonic hindlimb morphogenesis; IEA:Compara.
 GO:0048562; P:embryonic organ morphogenesis; IEA:Compara.
 GO:0048730; P:epidermis morphogenesis; IEA:Compara.
 GO:0030198; P:extracellular matrix organization; IEA:Compara.
 GO:0030900; P:forebrain development; IEA:Compara.
 GO:0007403; P:glial cell fate determination; IEA:Compara.
 GO:0060347; P:heart trabecula formation; IEA:Compara.
 GO:0030902; P:hindbrain development; IEA:Compara.
 GO:0043966; P:histone H3 acetylation; IEA:Compara.
 GO:0030216; P:keratinocyte differentiation; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0006346; P:methylation-dependent chromatin silencing; IEA:Compara.
 GO:0060766; P:negative regulation of androgen receptor signaling pathway; IEA:Compara.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0003407; P:neural retina development; IEA:Compara.
 GO:0006337; P:nucleosome disassembly; IEA:Compara.
 GO:0043923; P:positive regulation by host of viral transcription; IEA:Compara.
 GO:0043388; P:positive regulation of DNA binding; IEA:Compara.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0001570; P:vasculogenesis; IEA:Compara. 
Interpro
 IPR006576; BRK_domain.
 IPR001487; Bromodomain.
 IPR018359; Bromodomain_CS.
 IPR014978; Gln-Leu-Gln_QLQ.
 IPR013999; HAS_subgr.
 IPR014012; Helicase/SANT-assoc_DNA-bd.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR006562; HSA.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF07533; BRK
 PF00439; Bromodomain
 PF00271; Helicase_C
 PF07529; HSA
 PF08880; QLQ
 PF00176; SNF2_N 
SMART
 SM00592; BRK
 SM00297; BROMO
 SM00487; DEXDc
 SM00490; HELICc
 SM00573; HSA
 SM00951; QLQ 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51204; HSA
 PS51666; QLQ 
PRINTS
 PR00503; BROMODOMAIN.