CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014721
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rap1 GTPase-activating protein 2 
Protein Synonyms/Alias
 Rap1GAP2; GTPase-activating Rap/Ran-GAP domain-like protein 4 
Gene Name
 RAP1GAP2 
Gene Synonyms/Alias
 GARNL4; KIAA1039; RAP1GA2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
216IPLAGLSKLPSVPQIubiquitination[1, 2, 3]
268KFGVIYQKARQTLEEubiquitination[1]
424PSPPVFQKGPEFREFubiquitination[2, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state. 
Sequence Annotation
 DOMAIN 248 464 Rap-GAP.
 MOD_RES 7 7 Phosphoserine; by PKG/PRKG1; in vitro.
 MOD_RES 45 45 Phosphoserine.
 MOD_RES 49 49 Phosphothreonine.
 MOD_RES 544 544 Phosphoserine.
 MOD_RES 558 558 Phosphoserine.
 MOD_RES 564 564 Phosphoserine.
 MOD_RES 613 613 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; GTPase activation; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 730 AA 
Protein Sequence
MFGRKRSVSF GGFGWIDKTM LASLKVKKQE LANSSDATLP DRPLSPPLTA PPTMKSSEFF 60
EMLEKMQGIK LEEQKPGPQK NKDDYIPYPS IDEVVEKGGP YPQVILPQFG GYWIEDPENV 120
GTPTSLGSSI CEEEEEDNLS PNTFGYKLEC KGEARAYRRH FLGKDHLNFY CTGSSLGNLI 180
LSVKCEEAEG IEYLRVILRS KLKTVHERIP LAGLSKLPSV PQIAKAFCDD AVGLRFNPVL 240
YPKASQMIVS YDEHEVNNTF KFGVIYQKAR QTLEEELFGN NEESPAFKEF LDLLGDTITL 300
QDFKGFRGGL DVTHGQTGVE SVYTTFRDRE IMFHVSTKLP FTDGDAQQLQ RKRHIGNDIV 360
AIIFQEENTP FVPDMIASNF LHAYIVVQVE TPGTETPSYK VSVTAREDVP TFGPPLPSPP 420
VFQKGPEFRE FLLTKLTNAE NACCKSDKFA KLEDRTRAAL LDNLHDELHA HTQAMLGLGP 480
EEDKFENGGH GGFLESFKRA IRVRSHSMET MVGGQKKSHS GGIPGSLSGG ISHNSMEVTK 540
TTFSPPVVAA TVKNQSRSPI KRRSGLFPRL HTGSEGQGDS RARCDSTSST PKTPDGGHSS 600
QEIKSETSSN PSSPEICPNK EKPFMKLKEN GRAISRSSSS TSSVSSTAGE GEAMEEGDSG 660
GSQPSTTSPF KQEVFVYSPS PSSESPSLGA AATPIIMSRS PTDAKSRNSP RSNLKFRFDK 720
LSHASSGAGH 730 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR000331; Rap_GAP_dom. 
Pfam
 PF02145; Rap_GAP 
SMART
  
PROSITE
 PS50085; RAPGAP 
PRINTS