UniProt Accession

 RHOA_MOUSE; Q9QUI0; O88336 

Genbank Protein ID

 AF014371; AF178958; AF178959; AF178960; AF178961; AK077606; AK083624; BC068115 

Genbank Nucleotide ID

 AAC23710.1; AAD52675.1; AAD52676.1; AAD52677.1; AAD52678.1; BAC36896.1; BAC38971.1; AAH68115.1 

Protein Name

 Transforming protein RhoA 

Protein Synonyms/Alias

  

Gene Name

 Rhoa 

Gene Synonyms/Alias

 Arha; Arha2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
135	RRELAKMKQEPVKPE	acetylation	[1]
135	RRELAKMKQEPVKPE	ubiquitination	[2]
162	GYMECSAKTKDGVRE	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Required for the apical junction formation of keratinocyte cell-cell adhesion. 

Sequence Annotation

 NP_BIND 12 19 GTP (By similarity).
 NP_BIND 59 63 GTP (By similarity).
 NP_BIND 117 120 GTP (By similarity).
 MOTIF 34 42 Effector region (Potential).
 MOD_RES 188 188 Phosphoserine; by PKG/PRKG1 (By
 MOD_RES 190 190 Cysteine methyl ester (By similarity).
 LIPID 190 190 S-geranylgeranyl cysteine (By  

Keyword

 3D-structure; Cell cycle; Cell division; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Phosphoprotein; Prenylation; Proto-oncogene; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 193 AA 

Protein Sequence

Gene Ontology

 GO:0043296; C:apical junction complex; IEA:Compara.
 GO:0030424; C:axon; IEA:Compara.
 GO:0005938; C:cell cortex; ISS:UniProtKB.
 GO:0032154; C:cleavage furrow; IEA:UniProtKB-SubCell.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0030027; C:lamellipodium; IDA:MGI.
 GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0032587; C:ruffle membrane; IDA:MGI.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:MGI.
 GO:0030521; P:androgen receptor signaling pathway; IDA:MGI.
 GO:0043297; P:apical junction assembly; IDA:UniProtKB.
 GO:0000902; P:cell morphogenesis; IGI:MGI.
 GO:0007160; P:cell-matrix adhesion; IDA:MGI.
 GO:0021795; P:cerebral cortex cell migration; IMP:MGI.
 GO:0036089; P:cleavage furrow formation; ISS:UniProtKB.
 GO:0021861; P:forebrain radial glial cell differentiation; IMP:MGI.
 GO:0007229; P:integrin-mediated signaling pathway; TAS:MGI.
 GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; IEA:Compara.
 GO:0033144; P:negative regulation of intracellular steroid hormone receptor signaling pathway; IDA:MGI.
 GO:0043524; P:negative regulation of neuron apoptotic process; IMP:MGI.
 GO:0045665; P:negative regulation of neuron differentiation; IEA:Compara.
 GO:0048812; P:neuron projection morphogenesis; IEA:Compara.
 GO:0043931; P:ossification involved in bone maturation; IMP:BHF-UCL.
 GO:0030838; P:positive regulation of actin filament polymerization; IEA:Compara.
 GO:0045785; P:positive regulation of cell adhesion; IEA:Compara.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; ISS:UniProtKB.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara.
 GO:0045666; P:positive regulation of neuron differentiation; ISO:MGI.
 GO:0071803; P:positive regulation of podosome assembly; IGI:MGI.
 GO:0051496; P:positive regulation of stress fiber assembly; ISO:MGI.
 GO:0045727; P:positive regulation of translation; IEA:Compara.
 GO:0045907; P:positive regulation of vasoconstriction; IEA:Compara.
 GO:0051924; P:regulation of calcium ion transport; IEA:Compara.
 GO:0030334; P:regulation of cell migration; ISS:UniProtKB.
 GO:0050773; P:regulation of dendrite development; IEA:Compara.
 GO:2000177; P:regulation of neural precursor cell proliferation; IMP:MGI.
 GO:0033688; P:regulation of osteoblast proliferation; IMP:BHF-UCL.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0043200; P:response to amino acid stimulus; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0009612; P:response to mechanical stimulus; IEA:Compara.
 GO:0007266; P:Rho protein signal transduction; TAS:MGI.
 GO:0007519; P:skeletal muscle tissue development; IDA:MGI.
 GO:0090307; P:spindle assembly involved in mitosis; IEA:Compara.
 GO:0043149; P:stress fiber assembly; IDA:MGI.
 GO:0031098; P:stress-activated protein kinase signaling cascade; IEA:Compara.
 GO:0061383; P:trabecula morphogenesis; IMP:BHF-UCL. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 

Pfam

 PF00071; Ras 

SMART

 SM00174; RHO 

PROSITE

 PS51420; RHO 

PRINTS

 PR00449; RASTRNSFRMNG.