CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010975
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic initiation factor 4A 
Protein Synonyms/Alias
 eIF-4A; ATP-dependent RNA helicase eIF4A 
Gene Name
 eIF-4a 
Gene Synonyms/Alias
 eIF4A; l(2L)162; CG9075 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
52IYGYGFEKPSAIQQRacetylation[1]
190EMLSRGFKDQIQDVFacetylation[1]
235PVSILVKKEELTLEGacetylation[1]
313RDREVIMKQFRSGSSacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Involved in germ cell formation. 
Sequence Annotation
 DOMAIN 61 231 Helicase ATP-binding.
 DOMAIN 242 403 Helicase C-terminal.
 NP_BIND 74 81 ATP (By similarity).
 MOTIF 30 58 Q motif.
 MOTIF 179 182 DEAD box.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; Initiation factor; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 403 AA 
Protein Sequence
MDDRNEIPQD GPASMEPEGV IESTWHEVYD NFDDMNLREE LLRGIYGYGF EKPSAIQQRA 60
IIPCVRGRDV IAQAQSGTGK TATFSIAILQ QIDTSIRECQ ALILAPTREL ATQIQRVVMA 120
LGEYMKVHSH ACIGGTNVRE DARILESGCH VVVGTPGRVY DMINRKVLRT QYIKLFVLDE 180
ADEMLSRGFK DQIQDVFKML PPDVQVILLS ATMPPDVLEV SRCFMRDPVS ILVKKEELTL 240
EGIKQFYVNV KQENWKLGTL CDLYDTLSIT QSVIFCNTRR KVDQLTQEMS IHNFTVSAMH 300
GDMEQRDREV IMKQFRSGSS RVLITTDLLA RGIDVQQVSL VINYDLPSNR ENYIHRIGRG 360
GRFGRKGVAI NFITDDDRRI LKDIEQFYHT TIEEMPANIA DLI 403 
Gene Ontology
 GO:0005813; C:centrosome; IDA:FlyBase.
 GO:0005829; C:cytosol; ISS:FlyBase.
 GO:0016281; C:eukaryotic translation initiation factor 4F complex; ISS:FlyBase.
 GO:0005875; C:microtubule associated complex; IDA:FlyBase.
 GO:0072686; C:mitotic spindle; IDA:FlyBase.
 GO:0005634; C:nucleus; IC:FlyBase.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003724; F:RNA helicase activity; ISS:FlyBase.
 GO:0003743; F:translation initiation factor activity; ISS:FlyBase.
 GO:0051297; P:centrosome organization; IMP:FlyBase.
 GO:0048132; P:female germ-line stem cell division; IMP:FlyBase.
 GO:0030718; P:germ-line stem cell maintenance; IMP:FlyBase.
 GO:0007446; P:imaginal disc growth; TAS:FlyBase.
 GO:0002168; P:instar larval development; TAS:FlyBase.
 GO:0007067; P:mitosis; IMP:FlyBase.
 GO:0000022; P:mitotic spindle elongation; IMP:FlyBase.
 GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:FlyBase.
 GO:0006974; P:response to DNA damage stimulus; IMP:FlyBase. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS