CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005133
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 3 subunit A 
Protein Synonyms/Alias
 eIF3a; Centrosomin; Eukaryotic translation initiation factor 3 subunit 10; eIF-3-theta; eIF3 p167; eIF3 p180; eIF3 p185; p162 
Gene Name
 Eif3a 
Gene Synonyms/Alias
 Csma; Eif3; Eif3s10 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
23NEFLEVGKKQPALDVubiquitination[1]
24EFLEVGKKQPALDVLubiquitination[1]
45KKHRTWQKIHEPIMLubiquitination[1]
68LRKSHLAKEGLYQYKacetylation[2]
68LRKSHLAKEGLYQYKubiquitination[1]
75KEGLYQYKNICQQVNubiquitination[1, 3]
308HLSREMRKNLTQEEMubiquitination[1]
351MDGIIVEKQRRLATLubiquitination[1]
409KLCERVTKVLNWVREacetylation[2]
409KLCERVTKVLNWVREubiquitination[1]
420WVREQPEKEPELQQYubiquitination[1, 3]
559LAVNAYLKNSRKEHQacetylation[4]
559LAVNAYLKNSRKEHQubiquitination[1]
579RQTIEERKERLESLNubiquitination[1]
591SLNIQREKEELEQREubiquitination[1]
651KKTELGAKAFKDIDIubiquitination[1]
694RLKNQEKKIDYFERAubiquitination[1]
711LEEIPLIKSAYEEQRubiquitination[1]
775RQSVYEEKLKQFEERubiquitination[1, 3]
824RAEEQMLKEREERERubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
 Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A.
 Nature. 2012 Nov 22;491(7425):618-21. [PMID: 23086144]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 
Sequence Annotation
 DOMAIN 366 494 PCI.
 REPEAT 924 931 1; truncated.
 REPEAT 932 941 2.
 REPEAT 942 951 3; approximate.
 REPEAT 953 962 4.
 REPEAT 963 972 5.
 REPEAT 973 982 6.
 REPEAT 983 992 7.
 REPEAT 993 1002 8.
 REPEAT 1003 1012 9.
 REPEAT 1013 1022 10.
 REPEAT 1023 1032 11.
 REPEAT 1033 1042 12.
 REPEAT 1043 1052 13.
 REPEAT 1054 1063 14.
 REPEAT 1064 1073 15.
 REPEAT 1074 1083 16.
 REPEAT 1084 1093 17.
 REPEAT 1094 1103 18.
 REPEAT 1104 1113 19.
 REPEAT 1114 1123 20.
 REPEAT 1124 1133 21; approximate.
 REGION 664 835 Interaction with EIF3B (By similarity).
 REGION 924 1133 21 X 10 AA approximate tandem repeats of
 MOD_RES 68 68 N6-acetyllysine (By similarity).
 MOD_RES 492 492 Phosphoserine (By similarity).
 MOD_RES 1159 1159 Phosphoserine (By similarity).
 MOD_RES 1300 1300 Phosphoserine (By similarity).
 MOD_RES 1326 1326 Phosphoserine (By similarity).  
Keyword
 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Initiation factor; Nucleus; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; RNA editing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1344 AA 
Protein Sequence
MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD 60
LRKSHLAKEG LYQYKNICQQ VNIKSLEDVV RAYLKLAEEK TEAAKEESQQ MVLDIEDLDN 120
IQTPESVLLS AVSGEDTQDR TDRLLLTPWV KFLWESYRQC LDLLRNNSRV ERLYHDIAQQ 180
AFKFCLQYTR KAEFRKLCDN LRMHLSQIQR HHNQSTAINL NNPESQSMHL ETRLVQLDSA 240
ISMELWQEAF KAVEDIHGLF SLSKKPPKPQ LMANYYNKVS TVFWKSGNAL FHASTLHRLY 300
HLSREMRKNL TQEEMQRMST RVLLATLSIP ITPERTDIAR LLDMDGIIVE KQRRLATLLG 360
LQAPPTRIGL INDMVRFSVL QYVVPEVKDL YNWLEVEFNP LKLCERVTKV LNWVREQPEK 420
EPELQQYVPQ LQNNTILRLL QQVAQIYQSI EFSRLTSLVP FVDAFQLERA IVDAARHCDL 480
QVRIDHTSRT LSFGSDLNYA TREDAPVGPH LQSMPSEQIR NQLTAMSSVL AKALEVIRPA 540
HILQEKEEQH QLAVNAYLKN SRKEHQRILA RRQTIEERKE RLESLNIQRE KEELEQREAE 600
LQKVRKAEEE RLRQEAKERE KERILQEHEQ IKKKTVRERL EQIKKTELGA KAFKDIDIED 660
LEELDPDFIM AKQVEQLEKE KKELQERLKN QEKKIDYFER AKRLEEIPLI KSAYEEQRVK 720
DMDLWEQQEE ERITTMQLER EKALEHKNRM SRMLEDRDLF VMRLKAARQS VYEEKLKQFE 780
ERLAEERHSR LEDRKRQRKE ERKITYYREK EEEEQRRAEE QMLKEREERE RAERAKREEE 840
LREYQERVKK LEEVERKKRQ RELEIEERER RREEERRLGD DPLSRKDSRW GDRDSEGTWR 900
KGPEADSEWR RGPPEKEWRR ETRDDERPHR RDEDRLRRLG GDDEERESSL RPDDDRIPRR 960
GLDDDRGPRR GPDEDRFSRR GTDDDRPSWR NADDDRPPRR IGDDDRGSWR HTDDDRPPRR 1020
GLDDERGSWR TADEDRGPRR GMDDDRGPRR GGADDERSSW RNADDDRGPR RGMDDDRGPR 1080
RGLDDDRGPW RNAAEDRISR RGADDDRGPW RNMDDDRVPR RGDDARPGPW RPFVKPGGWR 1140
EKEKAREESW GPPRESRPSE EREWDRDKEK DRDNQDREEN DKDLERDRDR ERDGDREDRF 1200
RRPRDEGGWR RGPAEESSSW RDSSRRDDRD REDRRRDRDD RRDLRDLRDR RDLRDDRDRR 1260
GPPLRSEREE ASSWRRTDDR KDDRTEERDP PRRVPPPALS RDRERERERE GEKEKASWRA 1320
EKDRESLRRT KNETDEDGWT TVRR 1344 
Gene Ontology
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001732; P:formation of translation initiation complex; IDA:UniProtKB.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 
Interpro
 IPR027512; EIF3A.
 IPR000717; PCI_dom. 
Pfam
 PF01399; PCI 
SMART
 SM00088; PINT 
PROSITE
  
PRINTS