| Tag | Content |
|---|
| CPLM ID | CPLM-017891 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 15 |
Protein Synonyms/Alias | Deubiquitinating enzyme 15; Ubiquitin thioesterase 15; Ubiquitin-specific-processing protease 15 |
Gene Name | Usp15 |
Gene Synonyms/Alias | Kiaa0529 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 154 | VVTRRFSKADTIDTI | ubiquitination | [1] | | 193 | NTFEPLNKPDSTIQD | ubiquitination | [1] | | 228 | PRGPSTPKSPGASNF | ubiquitination | [1] | | 259 | NINNRNVKNSNYCLP | ubiquitination | [1] | | 363 | YVTPRAFKTQVGRFA | ubiquitination | [1] | | 605 | PRNNTEDKLYNLLLL | ubiquitination | [1] | | 758 | LDWDPDLKKRYFDEN | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling and NF-kappa-B pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal. Able to mediate deubiquitination of monoubiquitinated substrates as well as 'Lys-48'-linked polyubiquitin chains, protecting them against proteasomal degradation. Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP (By similarity). |
Sequence Annotation | DOMAIN 7 118 DUSP.
ACT_SITE 298 298 Nucleophile (By similarity).
ACT_SITE 891 891 Proton acceptor (By similarity).
MOD_RES 2 2 N-acetylalanine (By similarity).
MOD_RES 229 229 Phosphoserine (By similarity).
MOD_RES 242 242 Phosphoserine (By similarity).
MOD_RES 961 961 Phosphoserine (By similarity).
MOD_RES 965 965 Phosphoserine (By similarity). |
Keyword | Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 981 AA |
Protein Sequence | MAEGGAADLD TQRSDIATLL KTSLRKGDTW YLVDSRWFKQ WKKYVGFDSW DKYQMGDQNV 60
YPGPIDNSGL LKDGDAQSLK EHLIDELDYI LLPTEGWNKL VSWYTLMEGQ EPIARKVVEQ 120
GMFVKHCKVE VYLTELKLCE NGNMNNVVTR RFSKADTIDT IEKEIRKIFN IPDEKEARLW 180
NKYMSNTFEP LNKPDSTIQD AGLYQGQVLV IEQKNEDGTW PRGPSTPKSP GASNFSTLPK 240
ISPSSLSNNY NNINNRNVKN SNYCLPSYTA YKNYDYSEPG RNNEQPGLCG LSNLGNTCFM 300
NSAIQCLSNT PPLTEYFLND KYQEELNFDN PLGMRGEIAK SYAELIKQMW SGKFSYVTPR 360
AFKTQVGRFA PQFSGYQQQD CQELLAFLLD GLHEDLNRIR KKPYIQLKDA DGRPDKVVAE 420
EAWENHLKRN DSIIVDIFHG LFKSTLVCPE CAKISVTFDP FCYLTLPLPM KKERSLEVYL 480
VRMDPLAKPM QYKVIVPKIG NILDLCTALS ALSGVPADKM IVTDIYNHRF HRIFAVDENL 540
SSIMERDDIY VFEININRAE DTEHVVIPVC LREKFRHSSY THHTGSSLFG QPFLMAIPRN 600
NTEDKLYNLL LLRMCRYVKM STETEETDGH LRCCEDQNIN GNGPNGLHEE GSPSEMETDE 660
PDDESSQDQE LPSENENSQS EDSVGGDNDS ENGLCTEETC KGQLTGHKKR LFTFQFNNLG 720
NNDINYIKDD TSHIRFDDRQ LRLDERSFLA LDWDPDLKKR YFDENAAEDF EKHESVEYKP 780
PKRPFVKLKD CIELFTTKEK LGAEDPWYCP NCKEHQQATK KLDLWSLPPV LVVHLKRFSY 840
SRYMRDKLDT LVDFPISDLD MSEFLINPNA GPCRYNLIAV SNHYGGMGGG HYTAFAKNKD 900
DGKWYYFDDS SVSTASEDQI VSKAAYVLFY QRQDTFSGTG FFPLDRETKG ASAATGIPLE 960
SDEDSNDNDN DLENENCMHT N 981 |
Gene Ontology | GO:0005737; C:cytoplasm; ISS:UniProtKB. GO:0005634; C:nucleus; ISS:UniProtKB. GO:0004197; F:cysteine-type endopeptidase activity; ISS:UniProtKB. GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB. GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB. GO:0030509; P:BMP signaling pathway; ISS:UniProtKB. GO:0035520; P:monoubiquitinated protein deubiquitination; ISS:UniProtKB. GO:0060389; P:pathway-restricted SMAD protein phosphorylation; ISS:UniProtKB. GO:0007179; P:transforming growth factor beta receptor signaling pathway; ISS:UniProtKB. GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. |
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