CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004595
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CCAAT/enhancer-binding protein beta 
Protein Synonyms/Alias
 C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5 
Gene Name
 CEBPB 
Gene Synonyms/Alias
 LAP; TCF5; PP9092 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
174PPPPAELKAEPGFEPsumoylation[1]
260APAPSQVKSKAKKTVubiquitination[2]
265QVKSKAKKTVDKHSDacetylation[3]
269KAKKTVDKHSDEYKIacetylation[3]
332STLRNLFKQLPEPLLubiquitination[4, 5]
Reference
 [1] Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier (SUMO) family members, SUMO-2 and SUMO-3.
 Eaton EM, Sealy L.
 J Biol Chem. 2003 Aug 29;278(35):33416-21. [PMID: 12810706]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation (By similarity). Regulates the transcriptional induction of peroxisome proliferator-activated receptor gamma (PPARG). 
Sequence Annotation
 DOMAIN 271 334 bZIP.
 REGION 1 24 Required for Lys-174 sumoylation.
 REGION 275 295 Basic motif (By similarity).
 REGION 297 304 Leucine-zipper (By similarity).
 MOTIF 116 124 9aaTAD.
 MOD_RES 235 235 Phosphothreonine; by RPS6KA1.
 CROSSLNK 174 174 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Activator; Complete proteome; Differentiation; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 345 AA 
Protein Sequence
MQRLVAWDPA CLPLPPPPPA FKSMEVANFY YEADCLAAAY GGKAAPAAPP AARPGPRPPA 60
GELGSIGDHE RAIDFSPYLE PLGAPQAPAP ATATDTFEAA PPAPAPAPAS SGQHHDFLSD 120
LFSDDYGGKN CKKPAEYGYV SLGRLGAAKG ALHPGCFAPL HPPPPPPPPP AELKAEPGFE 180
PADCKRKEEA GAPGGGAGMA AGFPYALRAY LGYQAVPSGS SGSLSTSSSS SPPGTPSPAD 240
AKAPPTACYA GAAPAPSQVK SKAKKTVDKH SDEYKIRRER NNIAVRKSRD KAKMRNLETQ 300
HKVLELTAEN ERLQKKVEQL SRELSTLRNL FKQLPEPLLA SSGHC 345 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000790; C:nuclear chromatin; IEA:Compara.
 GO:0016363; C:nuclear matrix; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; ISS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0006953; P:acute-phase response; TAS:ProtInc.
 GO:0050873; P:brown fat cell differentiation; IEA:Compara.
 GO:0071230; P:cellular response to amino acid stimulus; IEA:Compara.
 GO:0001892; P:embryonic placenta development; IEA:Compara.
 GO:0006955; P:immune response; TAS:ProtInc.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0060644; P:mammary gland epithelial cell differentiation; IEA:Compara.
 GO:0033598; P:mammary gland epithelial cell proliferation; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0030182; P:neuron differentiation; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Compara.
 GO:0045408; P:regulation of interleukin-6 biosynthetic process; IEA:Compara.
 GO:0034976; P:response to endoplasmic reticulum stress; IDA:UniProtKB.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara. 
Interpro
 IPR004827; bZIP.
 IPR016468; CCAAT/enhancer-binding. 
Pfam
 PF07716; bZIP_2 
SMART
 SM00338; BRLZ 
PROSITE
 PS50217; BZIP
 PS00036; BZIP_BASIC 
PRINTS