CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014232
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Src substrate cortactin 
Protein Synonyms/Alias
  
Gene Name
 Cttn 
Gene Synonyms/Alias
 Ems1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
87ASHGYGGKFGVEQDRacetylation[1, 2, 3]
107VGHEYQSKLSKHCSQacetylation[4]
110EYQSKLSKHCSQVDSacetylation[4]
124SVRGFGGKFGVQMDRacetylation[1, 3, 4]
124SVRGFGGKFGVQMDRubiquitination[5]
144VGFEYQGKTEKHASQacetylation[2, 3, 4]
161YSSGFGGKYGVQADRacetylation[1, 3, 4, 6]
171VQADRVDKSAVGFDYacetylation[3]
171VQADRVDKSAVGFDYsuccinylation[3]
181VGFDYQGKTEKHESQacetylation[3]
189TEKHESQKDYSKGFGacetylation[1]
198YSKGFGGKYGIDKDKacetylation[1, 2, 3]
203GGKYGIDKDKVDKSAacetylation[1, 3]
205KYGIDKDKVDKSAVGacetylation[3]
235YVKGFGGKFGVQTDRacetylation[1, 2, 3, 4]
263LQLHESQKDYKTGFGacetylation[7]
272YKTGFGGKFGVQSERacetylation[1, 2, 3, 6, 7]
295DYKERLAKHESQQDYacetylation[3]
304ESQQDYAKGFGGKYGacetylation[2]
309YAKGFGGKYGVQKDRacetylation[1, 2, 3]
309YAKGFGGKYGVQKDRubiquitination[5]
319VQKDRMDKNASTFEEacetylation[1]
336QVPSAYQKTVPIEAVubiquitination[5]
346PIEAVTSKTSNIRANacetylation[3]
Reference
 [1] HDAC6 modulates cell motility by altering the acetylation level of cortactin.
 Zhang X, Yuan Z, Zhang Y, Yong S, Salas-Burgos A, Koomen J, Olashaw N, Parsons JT, Yang XJ, Dent SR, Yao TP, Lane WS, Seto E.
 Mol Cell. 2007 Jul 20;27(2):197-213. [PMID: 17643370]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Contributes to the organization of the actin cytoskeleton and cell structure. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases. 
Sequence Annotation
 REPEAT 80 116 Cortactin 1.
 REPEAT 117 153 Cortactin 2.
 REPEAT 154 190 Cortactin 3.
 REPEAT 191 227 Cortactin 4.
 REPEAT 228 264 Cortactin 5.
 REPEAT 265 301 Cortactin 6.
 REPEAT 302 324 Cortactin 7; truncated.
 DOMAIN 488 546 SH3.
 MOD_RES 87 87 N6-acetyllysine (By similarity).
 MOD_RES 198 198 N6-acetyllysine (By similarity).
 MOD_RES 235 235 N6-acetyllysine (By similarity).
 MOD_RES 272 272 N6-acetyllysine (By similarity).
 MOD_RES 304 304 N6-acetyllysine (By similarity).
 MOD_RES 309 309 N6-acetyllysine (By similarity).
 MOD_RES 401 401 Phosphothreonine.
 MOD_RES 405 405 Phosphoserine.
 MOD_RES 407 407 Phosphoserine.
 MOD_RES 417 417 Phosphoserine (By similarity).
 MOD_RES 418 418 Phosphoserine.
 MOD_RES 421 421 Phosphotyrosine (By similarity).
 MOD_RES 442 442 Phosphotyrosine (By similarity).
 MOD_RES 482 482 Phosphotyrosine; by SRC.
 MOD_RES 485 485 Phosphotyrosine; by SRC.  
Keyword
 3D-structure; Acetylation; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 546 AA 
Protein Sequence
MWKASAGHAV SITQDDGGAD DWETDPDFVN DVSEKEQRWG AKTVQGSGHQ EHINIHKLRE 60
NVFQEHQTLK EKELETGPKA SHGYGGKFGV EQDRMDRSAV GHEYQSKLSK HCSQVDSVRG 120
FGGKFGVQMD RVDQSAVGFE YQGKTEKHAS QKDYSSGFGG KYGVQADRVD KSAVGFDYQG 180
KTEKHESQKD YSKGFGGKYG IDKDKVDKSA VGFEYQGKTE KHESQKDYVK GFGGKFGVQT 240
DRQDKCALGW DHQEKLQLHE SQKDYKTGFG GKFGVQSERQ DSSAVGFDYK ERLAKHESQQ 300
DYAKGFGGKY GVQKDRMDKN ASTFEEVVQV PSAYQKTVPI EAVTSKTSNI RANFENLAKE 360
REQEDRRKAE AERAQRMAKE RQEQEEARRK LEEQARAKKQ TPPASPSPQP IEDRPPSSPI 420
YEDAAPFKAE PSYRGSEPEP EYSIEAAGIP EAGSQQGLTY TSEPVYETTE APGHYQAEDD 480
TYDGYESDLG ITAIALYDYQ AAGDDEISFD PDDIITNIEM IDDGWWRGVC KGRYGLFPAN 540
YVELRQ 546 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:MGI.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0043197; C:dendritic spine; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0001726; C:ruffle; IDA:UniProtKB. 
Interpro
 IPR015503; Cortactin.
 IPR003134; Hs1_Cortactin.
 IPR000108; p67phox.
 IPR001452; SH3_domain. 
Pfam
 PF02218; HS1_rep
 PF00018; SH3_1 
SMART
 SM00326; SH3 
PROSITE
 PS51090; CORTACTIN
 PS50002; SH3 
PRINTS
 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.