CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022894
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intraflagellar transport protein 172 homolog 
Protein Synonyms/Alias
  
Gene Name
 IFT172 
Gene Synonyms/Alias
 KIAA1179 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MHLKHLRTLLSsumoylation[1]
70GRKSYMVKGMAFSPDubiquitination[2]
102IGEDWGDKKVICNKFubiquitination[2]
103GEDWGDKKVICNKFIubiquitination[2]
499ELNETGHKLLFRDRKubiquitination[2, 3, 4, 5]
633AMWKTLSKLALEARQubiquitination[2]
656SALGQVAKARFLHETubiquitination[2, 6]
693ARLAMLEKNYKLAEMubiquitination[2]
775AAISLYLKAGLPAKAubiquitination[4]
781LKAGLPAKAARLVLTubiquitination[2]
821RAGDLFEKIHNPQKAubiquitination[2]
898LGARQWKKAIYILDLubiquitination[2]
942YTKGDRTKDAIDMYTubiquitination[2]
959GRWEQAHKLAMKCMRubiquitination[2]
1088QGGANAHKHVAYLWAubiquitination[2]
1214ARGALEEKDFQKAEGubiquitination[2]
1218LEEKDFQKAEGLLLRubiquitination[2, 4]
1250SDALRICKDYVPSQLubiquitination[2]
1301RAVDCYLKVRDSGNSubiquitination[2]
1313GNSGLAEKCWMKAAEubiquitination[2]
1482PQNFNIYKRIFTDMVubiquitination[2]
1720NKFLMAIKTSHSPVCubiquitination[2]
Reference
 [1] In vivo identification of sumoylation sites by a signature tag and cysteine-targeted affinity purification.
 Blomster HA, Imanishi SY, Siimes J, Kastu J, Morrice NA, Eriksson JE, Sistonen L.
 J Biol Chem. 2010 Jun 18;285(25):19324-9. [PMID: 20388717]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572
Functional Description
 Required for the maintenance and formation of cilia. Plays an indirect role in hedgehog (Hh) signaling, cilia being required for all activity of the hedgehog pathway (By similarity). 
Sequence Annotation
 REPEAT 14 53 WD 1.
 REPEAT 64 103 WD 2.
 REPEAT 110 148 WD 3.
 REPEAT 150 191 WD 4.
 REPEAT 195 233 WD 5.
 REPEAT 238 278 WD 6.
 REPEAT 284 323 WD 7.
 REPEAT 483 520 WD 8.
 REPEAT 521 559 WD 9.
 REPEAT 593 624 TPR 1.
 REPEAT 692 725 TPR 2.
 REPEAT 809 842 TPR 3.
 REPEAT 854 887 TPR 4.
 REPEAT 912 945 TPR 5.
 REPEAT 947 970 TPR 6.
 REPEAT 971 1004 TPR 7.
 REPEAT 1042 1075 TPR 8.
 REPEAT 1142 1175 TPR 9.
 REPEAT 1276 1309 TPR 10.
 REPEAT 1345 1378 TPR 11.
 REPEAT 1411 1445 TPR 12.
 REPEAT 1447 1477 TPR 13.
 REPEAT 1574 1607 TPR 14.
 MOD_RES 1 1 N-acetylmethionine.
 CROSSLNK 4 4 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Alternative splicing; Cell projection; Cilium; Complete proteome; Developmental protein; Isopeptide bond; Polymorphism; Reference proteome; Repeat; TPR repeat; Ubl conjugation; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1749 AA 
Protein Sequence
MHLKHLRTLL SPQDGAAKVT CMAWSQNNAK FAVCTVDRVV LLYDEHGERR DKFSTKPADM 60
KYGRKSYMVK GMAFSPDSTK IAIGQTDNII YVYKIGEDWG DKKVICNKFI QTSAVTCLQW 120
PAEYIIVFGL AEGKVRLANT KTNKSSTIYG TESYVVSLTT NCSGKGILSG HADGTIVRYF 180
FDDEGSGESQ GKLVNHPCPP YALAWATNSI VAAGCDRKIV AYGKEGHMLQ TFDYSRDPQE 240
REFTTAVSSP GGQSVVLGSY DRLRVFNWIP RRSIWEEAKP KEITNLYTIT ALAWKRDGSR 300
LCVGTLCGGV EQFDCCLRRS IYKNKFELTY VGPSQVIVKN LSSGTRVVLK SHYGYEVEEV 360
KILGKERYLV AHTSETLLLG DLNTNRLSEI AWQGSGGNEK YFFENENVCM IFNAGELTLV 420
EYGNNDTLGS VRTEFMNPHL ISVRINERCQ RGTEDNKKLA YLIDIKTIAI VDLIGGYNIG 480
TVSHESRVDW LELNETGHKL LFRDRKLRLH LYDIESCSKT MILNFCSYMQ WVPGSDVLVA 540
QNRNSLCVWY NIEAPERVTM FTIRGDVIGL ERGGGKTEVM VMEGVTTVAY TLDEGLIEFG 600
TAIDDGNYIR ATAFLETLEM TPETEAMWKT LSKLALEARQ LHIAERCFSA LGQVAKARFL 660
HETNEIADQV SREYGGEGTD FYQVRARLAM LEKNYKLAEM IFLEQNAVEE AMGMYQELHR 720
WDECIAVAEA KGHPALEKLR RSYYQWLMDT QQEERAGELQ ESQGDGLAAI SLYLKAGLPA 780
KAARLVLTRE ELLANTELVE HITAALIKGE LYERAGDLFE KIHNPQKALE CYRKGNAFMK 840
AVELARLAFP VEVVKLEEAW GDHLVQQKQL DAAINHYIEA RCSIKAIEAA LGARQWKKAI 900
YILDLQDRNT ASKYYPLVAQ HYASLQEYEI AEELYTKGDR TKDAIDMYTQ AGRWEQAHKL 960
AMKCMRPEDV SVLYITQAQE MEKQGKYREA ERLYVTVQEP DLAITMYKKH KLYDDMIRLV 1020
GKHHPDLLSD THLHLGKELE AEGRLQEAEY HYLEAQEWKA TVNMYRASGL WEEAYRVART 1080
QGGANAHKHV AYLWAKSLGG EAAVRLLNKL GLLEAAVDHA ADNCSFEFAF ELSRLALKHK 1140
TPEVHLKYAM FLEDEGKFEE AEAEFIRAGK PKEAVLMFVH NQDWEAAQRV AEAHDPDSVA 1200
EVLVGQARGA LEEKDFQKAE GLLLRAQRPG LALNYYKEAG LWSDALRICK DYVPSQLEAL 1260
QEEYEREATK KGARGVEGFV EQARHWEQAG EYSRAVDCYL KVRDSGNSGL AEKCWMKAAE 1320
LSIKFLPPQR NMEVVLAVGP QLIGIGKHSA AAELYLNLDL VKEAIDAFIE GEEWNKAKRV 1380
AKELDPRYED YVDQHYKEFL KNQGKVDSLV GVDVIAALDL YVEQGQWDKC IETATKQNYK 1440
ILHKYVALYA THLIREGSSA QALALYVQHG APANPQNFNI YKRIFTDMVS SPGTNCAEAY 1500
HSWADLRDVL FNLCENLVKS SEANSPAHEE FKTMLLIAHY YATRSAAQSV KQLETVAARL 1560
SVSLLRHTQL LPVDKAFYEA GIAAKAVGWD NMAFIFLNRF LDLTDAIEEG TLDGLDHSDF 1620
QDTDIPFEVP LPAKQHVPEA EREEVRDWVL TVSMDQRLEQ VLPRDERGAY EASLVAASTG 1680
VRALPCLITG YPILRNKIEF KRPGKAANKD NWNKFLMAIK TSHSPVCQDV LKFISQWCGG 1740
LPSTSFSFQ 1749 
Gene Ontology
 GO:0005929; C:cilium; ISS:UniProtKB.
 GO:0042384; P:cilium assembly; ISS:UniProtKB.
 GO:0007368; P:determination of left/right symmetry; IEA:Compara.
 GO:0009953; P:dorsal/ventral pattern formation; IEA:Compara.
 GO:0001841; P:neural tube formation; IEA:Compara.
 GO:0016485; P:protein processing; IEA:Compara.
 GO:0007224; P:smoothened signaling pathway; IEA:Compara. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
  
SMART
 SM00320; WD40 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS