UniProt Accession

 RAB43_HUMAN; Q86YS6; A8K4P9 

Genbank Protein ID

 AY166852; AK291014; CH471052; BC062319 

Genbank Nucleotide ID

 AAO17291.1; BAF83703.1; EAW79283.1; AAH62319.1 

Protein Name

 Ras-related protein Rab-43 

Protein Synonyms/Alias

 Ras-related protein Rab-41 

Gene Name

 RAB43 

Gene Synonyms/Alias

 RAB41 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
31	VGDASVGKTCVVQRF	ubiquitination	[1]
132	VQLLIGNKSDLSELR	ubiquitination	[2]
192	GGPLFSEKSPDHIQL	ubiquitination	[1, 2]
202	DHIQLNSKDIGEGWG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. 

Sequence Annotation

 NP_BIND 25 32 GTP.
 NP_BIND 73 77 GTP (By similarity).
 NP_BIND 131 134 GTP.
 NP_BIND 163 164 GTP.
 MOTIF 47 55 Effector region (By similarity).
 MOD_RES 82 82 Phosphothreonine (By similarity).
 MOD_RES 212 212 Cysteine methyl ester (By similarity).
 LIPID 210 210 S-geranylgeranyl cysteine (By
 LIPID 212 212 S-geranylgeranyl cysteine (By  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasmic vesicle; Golgi apparatus; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Phosphoprotein; Prenylation; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 212 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0045335; C:phagocytic vesicle; IDA:UniProtKB.
 GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:UniProtKB.
 GO:0007030; P:Golgi organization; IMP:UniProtKB.
 GO:0090382; P:phagosome maturation; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:InterPro.
 GO:0035526; P:retrograde transport, plasma membrane to Golgi; IMP:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003579; Small_GTPase_Rab_type. 

Pfam

 PF00071; Ras 

SMART

 SM00175; RAB 

PROSITE

 PS51419; RAB 

PRINTS

 PR00449; RASTRNSFRMNG.