CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022132
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sister chromatid cohesion protein PDS5 homolog B 
Protein Synonyms/Alias
 Androgen-induced proliferation inhibitor; Androgen-induced prostate proliferative shutoff-associated protein AS3 
Gene Name
 PDS5B 
Gene Synonyms/Alias
 APRIN; AS3; KIAA0979 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
121LKGLEDTKSPQFNRYubiquitination[1]
294EERLQVVKLLAKMFGubiquitination[2]
356KDLTEYLKVRSHDPEubiquitination[1, 3]
417MGLAQIYKKYALQSAacetylation[4, 5]
507NLLRHQVKDLLDLIKubiquitination[2, 3, 6]
574VSPTCSCKQAEGCVRubiquitination[2]
872TEQGKISKPDMSRLRubiquitination[2]
1089TYSLESPKDPVLPARubiquitination[7]
1103RFFTQPDKNFSNTKNubiquitination[2, 3]
1136NVLGAVNKPLSSAGKacetylation[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Plays a role in androgen-induced proliferative arrest in prostate cells. 
Sequence Annotation
 REPEAT 383 419 HEAT.
 DNA_BIND 1249 1261 A.T hook 1.
 DNA_BIND 1287 1299 A.T hook 2.
 DNA_BIND 1372 1384 A.T hook 3.
 MOD_RES 1136 1136 N6-acetyllysine.
 MOD_RES 1162 1162 Phosphoserine.
 MOD_RES 1166 1166 Phosphoserine.
 MOD_RES 1176 1176 Phosphoserine.
 MOD_RES 1182 1182 Phosphoserine.
 MOD_RES 1191 1191 Phosphoserine.
 MOD_RES 1257 1257 Phosphoserine.
 MOD_RES 1259 1259 Phosphoserine (By similarity).
 MOD_RES 1283 1283 Phosphoserine.
 MOD_RES 1334 1334 Phosphoserine.
 MOD_RES 1358 1358 Phosphoserine.
 MOD_RES 1367 1367 Phosphothreonine.
 MOD_RES 1370 1370 Phosphothreonine.
 MOD_RES 1379 1379 Phosphoserine.
 MOD_RES 1381 1381 Phosphothreonine.
 MOD_RES 1383 1383 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Cell cycle; Cell division; Complete proteome; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1447 AA 
Protein Sequence
MAHSKTRTND GKITYPPGVK EISDKISKEE MVRRLKMVVK TFMDMDQDSE EEKELYLNLA 60
LHLASDFFLK HPDKDVRLLV ACCLADIFRI YAPEAPYTSP DKLKDIFMFI TRQLKGLEDT 120
KSPQFNRYFY LLENIAWVKS YNICFELEDS NEIFTQLYRT LFSVINNGHN QKVHMHMVDL 180
MSSIICEGDT VSQELLDTVL VNLVPAHKNL NKQAYDLAKA LLKRTAQAIE PYITNFFNQV 240
LMLGKTSISD LSEHVFDLIL ELYNIDSHLL LSVLPQLEFK LKSNDNEERL QVVKLLAKMF 300
GAKDSELASQ NKPLWQCYLG RFNDIHVPIR LECVKFASHC LMNHPDLAKD LTEYLKVRSH 360
DPEEAIRHDV IVSIVTAAKK DILLVNDHLL NFVRERTLDK RWRVRKEAMM GLAQIYKKYA 420
LQSAAGKDAA KQIAWIKDKL LHIYYQNSID DRLLVERIFA QYMVPHNLET TERMKCLYYL 480
YATLDLNAVK ALNEMWKCQN LLRHQVKDLL DLIKQPKTDA SVKAIFSKVM VITRNLPDPG 540
KAQDFMKKFT QVLEDDEKIR KQLEVLVSPT CSCKQAEGCV REITKKLGNP KQPTNPFLEM 600
IKFLLERIAP VHIDTESISA LIKQVNKSID GTADDEDEGV PTDQAIRAGL ELLKVLSFTH 660
PISFHSAETF ESLLACLKMD DEKVAEAALQ IFKNTGSKIE EDFPHIRSAL LPVLHHKSKK 720
GPPRQAKYAI HCIHAIFSSK ETQFAQIFEP LHKSLDPSNL EHLITPLVTI GHIALLAPDQ 780
FAAPLKSLVA TFIVKDLLMN DRLPGKKTTK LWVPDEEVSP ETMVKIQAIK MMVRWLLGMK 840
NNHSKSGTST LRLLTTILHS DGDLTEQGKI SKPDMSRLRL AAGSAIVKLA QEPCYHEIIT 900
LEQYQLCALA INDECYQVRQ VFAQKLHKGL SRLRLPLEYM AICALCAKDP VKERRAHARQ 960
CLVKNINVRR EYLKQHAAVS EKLLSLLPEY VVPYTIHLLA HDPDYVKVQD IEQLKDVKEC 1020
LWFVLEILMA KNENNSHAFI RKMVENIKQT KDAQGPDDAK MNEKLYTVCD VAMNIIMSKS 1080
TTYSLESPKD PVLPARFFTQ PDKNFSNTKN YLPPEMKSFF TPGKPKTTNV LGAVNKPLSS 1140
AGKQSQTKSS RMETVSNASS SSNPSSPGRI KGRLDSSEMD HSENEDYTMS SPLPGKKSDK 1200
RDDSDLVRSE LEKPRGRKKT PVTEQEEKLG MDDLTKLVQE QKPKGSQRSR KRGHTASESD 1260
EQQWPEEKRL KEDILENEDE QNSPPKKGKR GRPPKPLGGG TPKEEPTMKT SKKGSKKKSG 1320
PPAPEEEEEE ERQSGNTEQK SKSKQHRVSR RAQQRAESPE SSAIESTQST PQKGRGRPSK 1380
TPSPSQPKKN VRVGRSKQAA TKENDSSEEV DVFQGSSPVD DIPQEETEEE EVSTVNVRRR 1440
SAKRERR 1447 
Gene Ontology
 GO:0000785; C:chromatin; IDA:UniProtKB.
 GO:0000775; C:chromosome, centromeric region; TAS:Reactome.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; TAS:ProtInc.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0000910; P:cytokinesis; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0007064; P:mitotic sister chromatid cohesion; IMP:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR017956; AT_hook_DNA-bd_motif. 
Pfam
  
SMART
 SM00384; AT_hook 
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS