UniProt Accession

 DDAH1_MOUSE; Q9CWS0; Q3UF65 

Genbank Protein ID

 AK010430; AK143507; AK148907; BC034505 

Genbank Nucleotide ID

 BAB26932.1; BAE25405.1; BAE28696.1; AAH34505.1 

Protein Name

 N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 

Protein Synonyms/Alias

 DDAH-1; Dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1 

Gene Name

 Ddah1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
106	RKEVDMMKEALEKLQ	acetylation	[1]
106	RKEVDMMKEALEKLQ	ubiquitination	[2]
159	EILADTFKDYAVSTV	ubiquitination	[2]
247	EEYPESAKVYEKLKD	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. 

Sequence Annotation

 ACT_SITE 173 173 Proton donor (By similarity).
 ACT_SITE 274 274 Nucleophile (By similarity).
 METAL 274 274 Zinc (By similarity).
 BINDING 30 30 Substrate; via carbonyl oxygen (By
 BINDING 73 73 Substrate (By similarity).
 BINDING 78 78 Substrate (By similarity).
 BINDING 79 79 Substrate (By similarity).
 BINDING 98 98 Substrate (By similarity).
 BINDING 145 145 Substrate (By similarity).
 BINDING 268 268 Substrate; via carbonyl oxygen (By
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 285 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0016597; F:amino acid binding; IEA:Compara.
 GO:0016403; F:dimethylargininase activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; ISA:MGI.
 GO:0006525; P:arginine metabolic process; IEA:Compara.
 GO:0000052; P:citrulline metabolic process; ISS:UniProtKB.
 GO:0006809; P:nitric oxide biosynthetic process; ISA:MGI.
 GO:0045766; P:positive regulation of angiogenesis; IEA:Compara.
 GO:0045429; P:positive regulation of nitric oxide biosynthetic process; IMP:BHF-UCL.
 GO:0017014; P:protein nitrosylation; ISA:MGI.
 GO:0003073; P:regulation of systemic arterial blood pressure; IMP:BHF-UCL. 

Interpro

 IPR003198; Amidino_trans. 

Pfam

 PF02274; Amidinotransf 

SMART

  

PROSITE

  

PRINTS