CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-035972
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 DNA polymerase kappa 
Protein Synonyms/Alias
  
Gene Name
 POLK 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
38LDKEKINKIIMEATKubiquitination[1]
45KIIMEATKGSRFYGNubiquitination[1]
55RFYGNELKKEKQVNQubiquitination[1]
56FYGNELKKEKQVNQRubiquitination[1]
71IENMMQQKAQITSQQubiquitination[1]
158AMPGFIAKRLCPQLIubiquitination[1]
173IVPPNFDKYRAVSKEubiquitination[1, 2]
224DKRRYFIKMGSSVENubiquitination[1]
236VENDNPGKEVNKLSEubiquitination[1]
240NPGKEVNKLSEHERSubiquitination[1]
326LAKVCSDKNKPNGQYubiquitination[1]
347QAVMDFIKDLPIRKVubiquitination[1]
359RKVSGIGKVTEKMLKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 427 AA 
Protein Sequence
MDSTKEKCDS YKDDLLLRMG LNDNKAGMEG LDKEKINKII MEATKGSRFY GNELKKEKQV 60
NQRIENMMQQ KAQITSQQLR KAQLQVDRFA MELEQSRNLS NTIVHIDMDA FYAAVEMRDN 120
PELKDKPIAV GSMSMLSTSN YHARRFGVRA AMPGFIAKRL CPQLIIVPPN FDKYRAVSKE 180
VKEILADYDP NFMAMSLDEA YLNITKHLEE RQNWPEDKRR YFIKMGSSVE NDNPGKEVNK 240
LSEHERSISP LLFEESPSDV QPPGDPFQVN FEEQNNPQIL QNSVVFGTSA QEVVKEIRFR 300
IEQKTTLTAS AGIAPNTMLA KVCSDKNKPN GQYQILPNRQ AVMDFIKDLP IRKVSGIGKV 360
TEKMLKALGI ITCTELYQQR ALLSLLFSET SWHYFLHISL GLGSTHLTRD GERKSMSVER 420
VELLPLS 427 
Gene Ontology
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
 GO:0006281; P:DNA repair; IEA:InterPro. 
Interpro
 IPR001126; DNA_repair_prot_UmuC-like.
 IPR017963; DNA_repair_prot_UmuC-like_N.
 IPR024728; PolY_HhH_motif. 
Pfam
 PF00817; IMS
 PF11798; IMS_HHH 
SMART
  
PROSITE
 PS50173; UMUC 
PRINTS