UniProt Accession

 THTM_MOUSE; Q99J99 

Genbank Protein ID

 BC004079 

Genbank Nucleotide ID

 AAH04079.1 

Protein Name

 3-mercaptopyruvate sulfurtransferase 

Protein Synonyms/Alias

 MST 

Gene Name

 Mpst 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
30	PRSSQPLKLLDASWY	ubiquitination	[1]
40	DASWYLPKLGRDARR	acetylation	[2]
164	QLDPSFIKTHEDILE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions. 

Sequence Annotation

 DOMAIN 25 144 Rhodanese 1.
 DOMAIN 174 288 Rhodanese 2.
 REGION 145 160 Hinge.
 ACT_SITE 248 248 Cysteine persulfide intermediate (By
 BINDING 188 188 Substrate (By similarity).
 DISULFID 264 264 Interchain (with C-264); redox-active (By  

Keyword

 Cell junction; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Mitochondrion; Redox-active center; Reference proteome; Repeat; Synapse; Synaptosome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 297 AA 

Protein Sequence

Gene Ontology

 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0043005; C:neuron projection; IDA:UniProtKB.
 GO:0045202; C:synapse; IEA:UniProtKB-KW.
 GO:0016784; F:3-mercaptopyruvate sulfurtransferase activity; IEA:EC.
 GO:0004792; F:thiosulfate sulfurtransferase activity; IEA:InterPro.
 GO:0070814; P:hydrogen sulfide biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR001763; Rhodanese-like_dom.
 IPR001307; Thiosulphate_STrfase_CS. 

Pfam

 PF00581; Rhodanese 

SMART

 SM00450; RHOD 

PROSITE

 PS00380; RHODANESE_1
 PS00683; RHODANESE_2
 PS50206; RHODANESE_3 

PRINTS