CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004434
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 
Protein Synonyms/Alias
 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex 
Gene Name
 odhB 
Gene Synonyms/Alias
 citM; BSU19360 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
185KAQQSFDKPVEVQKMacetylation[1]
308LTFAGIEKEIGELAKacetylation[1]
394DHRIVDGKEAVGFLVacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). 
Sequence Annotation
 DOMAIN 1 75 Lipoyl-binding.
 ACT_SITE 388 388 By similarity.
 ACT_SITE 392 392 By similarity.
 MOD_RES 42 42 N6-lipoyllysine (Potential).  
Keyword
 Acyltransferase; Complete proteome; Lipoyl; Reference proteome; Transferase; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 417 AA 
Protein Sequence
MAEIKVPELA ESISEGTIAQ WLKQPGDYVE QGEYLLELET DKVNVELTAE ESGVLQEVLK 60
DSGDTVQVGE IIGTISEGAG ESSAPAPTEK TESKESVKEE KQAEPAAQEV SEEAQSEAKS 120
RTIASPSARK LAREKGIDLS QVPTGDPLGR VRKQDVEAYE KPASKPAPQQ KQQPQAQKAQ 180
QSFDKPVEVQ KMSRRRQTIA KRLVEVQQTS AMLTTFNEVD MTAVMNLRKR RKDQFFEQNE 240
VKLGFMSFFT KAVVAALKKY PLLNAEIQGD ELIVKKFYDI GIAVAADEGL VVPVVRDADR 300
LTFAGIEKEI GELAKKARNN KLTLSELQGG SFTITNGGTF GSLMSTPILN SPQVGILGMH 360
KIQLRPVAID EERFENRPMM YIALSYDHRI VDGKEAVGFL VTIKNLLEDP EQLLLEG 417 
Gene Ontology
 GO:0045252; C:oxoglutarate dehydrogenase complex; IEA:InterPro.
 GO:0004149; F:dihydrolipoyllysine-residue succinyltransferase activity; IEA:EC.
 GO:0033512; P:L-lysine catabolic process to acetyl-CoA via saccharopine; IEA:UniProtKB-UniPathway.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 
Interpro
 IPR003016; 2-oxoA_DH_lipoyl-BS.
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR004167; E3-bd.
 IPR011053; Single_hybrid_motif.
 IPR006255; SucB. 
Pfam
 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl
 PF02817; E3_binding 
SMART
  
PROSITE
 PS50968; BIOTINYL_LIPOYL
 PS00189; LIPOYL 
PRINTS