CPLM-009070

Genbank Nucleotide ID

3-keto-steroid reductase

Protein Synonyms/Alias

17-beta-hydroxysteroid dehydrogenase 7; 17-beta-HSD 7; Estradiol 17-beta-dehydrogenase 7

Homo sapiens (Human)

Position	Peptide	Type	References
176	TSSRSARKSNFSLED	ubiquitination	[1, 2, 3]
188	LEDFQHSKGKEPYSS	ubiquitination	[1]
197	KEPYSSSKYATDLLS	ubiquitination	[2]
301	RNYIMTQKMDLDEDT	ubiquitination	[1, 4]
311	LDEDTAEKFYQKLLE	ubiquitination	[1, 2, 3, 4]
315	TAEKFYQKLLELEKH	ubiquitination	[1, 2, 4, 5, 6, 7]
321	QKLLELEKHIRVTIQ	acetylation	[8]
321	QKLLELEKHIRVTIQ	ubiquitination	[2, 3]
329	HIRVTIQKTDNQARL	ubiquitination	[1, 2, 3, 4, 6]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

Responsible for the reduction of the keto group on the C-3 of sterols.

NP_BIND 8 15 NAD (Potential).
ACT_SITE 193 193 Proton acceptor (By similarity).
BINDING 171 171 Substrate (By similarity).
CARBOHYD 37 37 N-linked (GlcNAc...) (Potential).
CARBOHYD 178 178 N-linked (GlcNAc...) (Potential).
CARBOHYD 229 229 N-linked (GlcNAc...) (Potential).

Alternative splicing; Cell membrane; Complete proteome; Glycoprotein; Lipid biosynthesis; Lipid metabolism; Membrane; NAD; NADP; Oxidoreductase; Reference proteome; Steroid biosynthesis; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
GO:0000253; F:3-keto sterol reductase activity; IEA:EC.
GO:0004303; F:estradiol 17-beta-dehydrogenase activity; TAS:ProtInc.
GO:0006695; P:cholesterol biosynthetic process; TAS:Reactome.
GO:0006703; P:estrogen biosynthetic process; IEA:UniProtKB-UniPathway.

IPR002198; DH_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DH.
IPR016040; NAD(P)-bd_dom.