UniProt Accession

 HYES_RAT; P80299 

Genbank Protein ID

 X65083; X60328 

Genbank Nucleotide ID

 CAA46211.1; CAA42898.1 

Protein Name

 Bifunctional epoxide hydrolase 2 

Protein Synonyms/Alias

 Cytosolic epoxide hydrolase 2; CEH; Epoxide hydratase; Soluble epoxide hydrolase; SEH; Lipid-phosphate phosphatase 

Gene Name

 Ephx2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
191	DDFGSNLKPARDMGM	acetylation	[1]
542	QILIKWLKTEIQNPS	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. 

Sequence Annotation

 REGION 1 224 Phosphatase.
 REGION 123 124 Phosphate binding (By similarity).
 REGION 233 554 Epoxide hydrolase.
 MOTIF 552 554 Microbody targeting signal (Potential).
 ACT_SITE 333 333 Nucleophile.
 ACT_SITE 465 465 Proton donor (By similarity).
 ACT_SITE 523 523 Proton acceptor.
 METAL 9 9 Magnesium (By similarity).
 METAL 11 11 Magnesium (By similarity).
 METAL 185 185 Magnesium (By similarity).
 BINDING 381 381 Substrate (By similarity).
 LIPID 521 521 S-(15-deoxy-Delta12,14-prostaglandin J2-  

Keyword

 Aromatic hydrocarbons catabolism; Complete proteome; Cytoplasm; Detoxification; Direct protein sequencing; Hydrolase; Lipid metabolism; Lipoprotein; Magnesium; Metal-binding; Multifunctional enzyme; Peroxisome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 554 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005777; C:peroxisome; IDA:RGD.
 GO:0033885; F:10-hydroxy-9-(phosphonooxy)octadecanoate phosphatase activity; IEA:EC.
 GO:0003869; F:4-nitrophenylphosphatase activity; IDA:RGD.
 GO:0004301; F:epoxide hydrolase activity; IDA:RGD.
 GO:0042577; F:lipid phosphatase activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; IMP:RGD.
 GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
 GO:0043651; P:linoleic acid metabolic process; IMP:RGD.
 GO:0046839; P:phospholipid dephosphorylation; ISS:UniProtKB.
 GO:0045777; P:positive regulation of blood pressure; IMP:RGD.
 GO:0002539; P:prostaglandin production involved in inflammatory response; IMP:RGD.
 GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
 GO:0019233; P:sensory perception of pain; IMP:RGD. 

Interpro

 IPR000073; AB_hydrolase_1.
 IPR000639; Epox_hydrolase-like.
 IPR023214; HAD-like_dom.
 IPR006402; HAD-SF_hydro_IA_v3.
 IPR011945; HAD-SF_ppase_IA/epoxid_hydro_N.
 IPR023198; PGP_dom2. 

Pfam

 PF00561; Abhydrolase_1
 PF13419; HAD_2 

SMART

  

PROSITE

  

PRINTS

 PR00111; ABHYDROLASE.
 PR00412; EPOXHYDRLASE.