CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001077
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor Sp3 
Protein Synonyms/Alias
  
Gene Name
 Sp3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
6**MTAPEKPVKQEEMacetylation[1]
553SPADIRIKEEEPDPEacetylation[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Sp3 is involved in the regulation of SOCS3 gene expression.
 Ehlting C, Häussinger D, Bode JG.
 Biochem J. 2005 May 1;387(Pt 3):737-45. [PMID: 15554904] 
Functional Description
 Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping (By similarity). 
Sequence Annotation
 ZN_FING 623 647 C2H2-type 1.
 ZN_FING 653 677 C2H2-type 2.
 ZN_FING 683 705 C2H2-type 3.
 REGION 140 239 Transactivation domain (Gln-rich) (By
 REGION 352 501 Transactivation domain (Gln-rich) (By
 REGION 536 622 Repressor domain (By similarity).
 MOD_RES 72 72 Phosphoserine (By similarity).
 MOD_RES 553 553 N6-acetyllysine; alternate.
 MOD_RES 565 565 Phosphoserine (By similarity).
 MOD_RES 648 648 Phosphoserine (By similarity).
 CROSSLNK 122 122 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 553 553 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Activator; Alternative splicing; Complete proteome; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 783 AA 
Protein Sequence
MTAPEKPVKQ EEMAALDVDG GGGGGGHGEY LQQQQQQQQQ HGNGAAAAAA QDTQPSPLAL 60
LAATCSKIGP PSPGDDDEEA AVAAAAGVPA AAAGATGDLA SAQLGGAPNR WEVLSATPTT 120
IKDEAGNLVQ IPGAATSSGQ YVLPLQNLQN QQIFSVAPGS DSSNGTVSNV QYQVIPQIQS 180
TDAQQVQIGF TGSSDNGGIN QENSQIQIIP GSNQTLLASG TPPANIQNLI PQTGQVQVQG 240
VAIGGSSFPG QTQVVANVPL GLPGNITFVP INSVDLDSLG LSGSSQTMTA GINADGHLIN 300
TGQAMDSSDN SERTGERVSP DVNETNADTD LFVPTSSSSQ LPVTIDSTGI LQQNTNSLTT 360
TSGQVHSSDL QGNYIQSPVS EETQAQNIQV STAQPVVQHL QLQDSQQPTS QAQIVQGITP 420
QTIHGVQASG QNISQQALQN LQLQLNPGTF LIQAQTVTPS GQITWQTFQV QGVQNLQNLQ 480
IQNTAAQQIT LTPVQTLTLG QVAAGGALTS TPVSLSTGQL PNLQTVTVNS IDSTGIQLHP 540
GENADSPADI RIKEEEPDPE EWQLSGDSTL NTNDLTHLRV QVVDEEGDQQ HQEGKRLRRV 600
ACTCPNCKEG GGRGTNLGKK KQHICHIPGC GKVYGKTSHL RAHLRWHSGE RPFICNWMFC 660
GKRFTRSDEL QRHRRTHTGE KKFVCPECSK RFMRSDHLAK HIKTHQNKKV IHSSSTVLAS 720
VEAGRDDALI TAGGTTLILA NIQQGSVSGI GTVNTSATSN QDILTNTEIP LQLVTVSGNE 780
TME 783 
Gene Ontology
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0017053; C:transcriptional repressor complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0000987; F:core promoter proximal region sequence-specific DNA binding; IDA:MGI.
 GO:0003690; F:double-stranded DNA binding; IDA:MGI.
 GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:UniProtKB.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IDA:MGI.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030183; P:B cell differentiation; IMP:MGI.
 GO:0060216; P:definitive hemopoiesis; IGI:MGI.
 GO:0048596; P:embryonic camera-type eye morphogenesis; IGI:MGI.
 GO:0001892; P:embryonic placenta development; IGI:MGI.
 GO:0060136; P:embryonic process involved in female pregnancy; IGI:MGI.
 GO:0048706; P:embryonic skeletal system development; IGI:MGI.
 GO:0043353; P:enucleate erythrocyte differentiation; IGI:MGI.
 GO:0030851; P:granulocyte differentiation; IMP:MGI.
 GO:0001889; P:liver development; IGI:MGI.
 GO:0030324; P:lung development; IGI:MGI.
 GO:0030219; P:megakaryocyte differentiation; IGI:MGI.
 GO:0030224; P:monocyte differentiation; IMP:MGI.
 GO:0001779; P:natural killer cell differentiation; IMP:MGI.
 GO:0001503; P:ossification; IGI:MGI.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0030217; P:T cell differentiation; IMP:MGI.
 GO:0001829; P:trophectodermal cell differentiation; IGI:MGI. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS