CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023510
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RanBP-type and C3HC4-type zinc finger-containing protein 1 
Protein Synonyms/Alias
 Heme-oxidized IRP2 ubiquitin ligase 1 homolog; HOIL-1; UbcM4-interacting protein 28; Ubiquitin-conjugating enzyme 7-interacting protein 3 
Gene Name
 Rbck1 
Gene Synonyms/Alias
 Rbck; Ubce7ip3; Uip28 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
158MLEDLGFKDLTLQSRubiquitination[1]
252LRQYQQRKQQQQEGNubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF- kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF- RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity). 
Sequence Annotation
 DOMAIN 55 119 Ubiquitin-like.
 ZN_FING 188 220 RanBP2-type.
 ZN_FING 280 325 RING-type 1.
 ZN_FING 360 409 IBR-type.
 ZN_FING 435 461 RING-type 2.
 REGION 1 268 Interaction with TAB2 (By similarity).
 REGION 1 218 Interaction with IRF3 (By similarity).
 REGION 69 131 Interaction with RNF31 (By similarity).
 MOD_RES 328 328 Phosphotyrosine.  
Keyword
 3D-structure; Coiled coil; Complete proteome; Ligase; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 508 AA 
Protein Sequence
MDEKTKKAEE MALSLARAVA GGDEQAAIKY ATWLAEQRVP LRVQVKPEVS PTQDIRLCVS 60
VEDAYMHTVT IWLTVRPDMT VASLKDMVFL DYGFPPSLQQ WVVGQRLARD QETLHSHGIR 120
RNGDGAYLYL LSARNTSLNP QELQRQRQLR MLEDLGFKDL TLQSRGPLEP VLPKPRTNQE 180
PGQPDAAPES PPVGWQCPGC TFINKPTRPG CEMCCRARPE TYQIPASYQP DEEERARLAG 240
EEEALRQYQQ RKQQQQEGNY LQHVQLEQRS LVLNTEPTEC PVCYSVLAPG EAVVLRECLH 300
TFCRECLQGT IRNSQEAEVA CPFIDSTYSC PGKLLEREIR ALLSPEDYQR FLDLGVSIAE 360
NRSTLSYHCK TPDCRGWCFF EDDVNEFTCP VCTRVNCLLC KAIHEHMNCR EYQDDLALRA 420
QNDVAARQTT EMLKVMLQQG EAMHCPQCRI VVQKKDGCDW IRCTVCHTEI CWVTKGPRWG 480
PGGPGDTSGG CRCRVNGIPC HPSCQNCH 508 
Gene Ontology
 GO:0071797; C:LUBAC complex; ISS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0043130; F:ubiquitin binding; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IMP:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0042346; P:positive regulation of NF-kappaB import into nucleus; IMP:UniProtKB.
 GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0097039; P:protein linear polyubiquitination; ISS:UniProtKB.
 GO:0050852; P:T cell receptor signaling pathway; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:InterPro. 
Interpro
 IPR026261; RBCK1.
 IPR019955; Ubiquitin_supergroup.
 IPR002867; Znf_C6HC.
 IPR001876; Znf_RanBP2.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF01485; IBR
 PF13639; zf-RING_2 
SMART
 SM00184; RING
 SM00547; ZnF_RBZ 
PROSITE
 PS50053; UBIQUITIN_2
 PS50119; ZF_BBOX
 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS