CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008408
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Estradiol 17-beta-dehydrogenase 2 
Protein Synonyms/Alias
 17-beta-hydroxysteroid dehydrogenase type 2; 17-beta-HSD 2; Testosterone 17-beta-dehydrogenase 
Gene Name
 Hsd17b2 
Gene Synonyms/Alias
 Edh17b2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
101GFGHGLAKHLDKLGFubiquitination[1]
142VLQMDVTKPEQIKDAubiquitination[1]
147VTKPEQIKDAHSKVTacetylation[2]
147VTKPEQIKDAHSKVTubiquitination[1]
152QIKDAHSKVTEKIQDacetylation[3]
262GVKVVTIKPGGFKTNubiquitination[1]
279GSQDIWDKMEKEILDacetylation[2, 3]
279GSQDIWDKMEKEILDubiquitination[1]
290EILDHFSKDIQENYGubiquitination[1]
305QDYVHTQKLIIPTLKubiquitination[1]
312KLIIPTLKERSNPDIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654
Functional Description
 Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH (By similarity). 
Sequence Annotation
 NP_BIND 83 112 NAD (By similarity).
 ACT_SITE 233 233 Proton acceptor (By similarity).
 BINDING 220 220 Substrate (By similarity).  
Keyword
 Complete proteome; Lipid biosynthesis; Lipid metabolism; Membrane; NAD; Oxidoreductase; Reference proteome; Signal-anchor; Steroid biosynthesis; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 381 AA 
Protein Sequence
MSPFASESAW LCLAAAAVLG GTLLCGCRSG RQLRSQAVCL AGLWGGACLL SLSLLCTLFL 60
LSVACFLLLY MSSSDQDLLP VDQKAVLVTG ADSGFGHGLA KHLDKLGFTV FAGVLDKEGP 120
GAEELRKHCS ERLSVLQMDV TKPEQIKDAH SKVTEKIQDK GLWAVVNNAG VFHLPIDGEL 180
IPMSIYRKCM AVNFFGTVEV TKAFLPLLRK SKGRLVNVSS MGGTVPLQMT SAYAATKAAL 240
TMFSTIIRQE LDKWGVKVVT IKPGGFKTNI TGSQDIWDKM EKEILDHFSK DIQENYGQDY 300
VHTQKLIIPT LKERSNPDIT PVLRDIQHAI SARNPSSFYY PGRMAYLWVC LAAYCPTSLL 360
DYVIKKGFYP QPTPRALRTV H 381 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IEA:EC.
 GO:0050327; F:testosterone 17-beta-dehydrogenase (NAD+) activity; IEA:Compara.
 GO:0047035; F:testosterone dehydrogenase (NAD+) activity; IEA:EC.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0001890; P:placenta development; IMP:MGI.
 GO:0032526; P:response to retinoic acid; IEA:Compara.
 GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW. 
Interpro
 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 
Pfam
 PF00106; adh_short 
SMART
  
PROSITE
 PS00061; ADH_SHORT 
PRINTS
 PR00081; GDHRDH.
 PR00080; SDRFAMILY.