UniProt Accession

 MTAP_MOUSE; Q9CQ65; Q3TJS4 

Genbank Protein ID

 AB056100; AK005064; AK011421; AK167319; BC003858 

Genbank Nucleotide ID

 BAB32865.1; BAB23788.1; BAB27609.1; BAE39421.1; AAH03858.1 

Protein Name

 S-methyl-5'-thioadenosine phosphorylase 

Protein Synonyms/Alias

 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; MTAPase 

Gene Name

 Mtap 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
32	ILEGRTEKYVDTPFG	acetylation	[1]
40	YVDTPFGKPSDALIL	acetylation	[1, 2]
40	YVDTPFGKPSDALIL	ubiquitination	[3]
49	SDALILGKIKNVDCV	acetylation	[1, 4]
49	SDALILGKIKNVDCV	succinylation	[4]
51	ALILGKIKNVDCVLL	acetylation	[1, 4]
51	ALILGKIKNVDCVLL	succinylation	[4]
71	QHTIMPSKVNYQANI	ubiquitination	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Catalyzes the reversible phosphorylation of S-methyl-5'- thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S- adenosylmethionine. Has broad substrate specificity with 6- aminopurine nucleosides as preferred substrates (By similarity). 

Sequence Annotation

 REGION 60 61 Phosphate binding (By similarity).
 REGION 93 94 Phosphate binding (By similarity).
 REGION 220 222 Substrate binding (By similarity).
 BINDING 18 18 Phosphate (By similarity).
 BINDING 196 196 Substrate; via amide nitrogen (By
 BINDING 197 197 Phosphate (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Glycosyltransferase; Nucleus; Purine salvage; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 283 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004645; F:phosphorylase activity; IEA:InterPro.
 GO:0017061; F:S-methyl-5-thioadenosine phosphorylase activity; IEA:HAMAP.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; IEA:HAMAP.
 GO:0006166; P:purine ribonucleoside salvage; IEA:UniProtKB-KW.
 GO:0033574; P:response to testosterone stimulus; IEA:Compara. 

Interpro

 IPR010044; MTAP.
 IPR000845; Nucleoside_phosphorylase_d.
 IPR001369; PNP/MTAP.
 IPR018099; Purine_phosphorylase-2_CS. 

Pfam

 PF01048; PNP_UDP_1 

SMART

  

PROSITE

 PS01240; PNP_MTAP_2 

PRINTS