CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009723
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 5A-1 
Protein Synonyms/Alias
 eIF-5A-1; eIF-5A1; Eukaryotic initiation factor 5A isoform 1; eIF-5A; Rev-binding factor; eIF-4D 
Gene Name
 EIF5A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
57MQCSALRKNGFVVLKubiquitination[1, 2, 3]
64KNGFVVLKGRPCKIVubiquitination[2]
69VLKGRPCKIVEMSTSubiquitination[1, 2]
97GIDIFTGKKYEDICPubiquitination[1, 2, 3]
98IDIFTGKKYEDICPSubiquitination[2, 3, 4]
115NMDVPNIKRNDFQLIubiquitination[1, 2, 3, 4]
151LPEGDLGKEIEQKYDubiquitination[2, 3]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. 
Sequence Annotation
 REGION 20 90 DOHH-binding.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 47 47 N6-acetyllysine.
 MOD_RES 50 50 Hypusine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; Endoplasmic reticulum; Hypusine; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Protein biosynthesis; Protein transport; Reference proteome; RNA-binding; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 154 AA 
Protein Sequence
MCGTGGTDSK TRRPPHRASF LKRLESKPLK MADDLDFETG DAGASATFPM QCSALRKNGF 60
VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ 120
LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK 180
AMAK 184 
Gene Ontology
 GO:0005642; C:annulate lamellae; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005643; C:nuclear pore; IDA:UniProtKB.
 GO:0043022; F:ribosome binding; IEA:InterPro.
 GO:0003746; F:translation elongation factor activity; ISS:UniProtKB.
 GO:0017070; F:U6 snRNA binding; IDA:UniProtKB.
 GO:0006917; P:induction of apoptosis; IDA:UniProtKB.
 GO:0006406; P:mRNA export from nucleus; IMP:UniProtKB.
 GO:0008612; P:peptidyl-lysine modification to hypusine; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; IGI:UniProtKB.
 GO:0045901; P:positive regulation of translational elongation; ISS:UniProtKB.
 GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0006611; P:protein export from nucleus; IMP:UniProtKB.
 GO:0006452; P:translational frameshifting; IEA:InterPro. 
Interpro
 IPR005824; KOW.
 IPR012340; NA-bd_OB-fold.
 IPR014722; Rib_L2_dom2.
 IPR019769; Trans_elong_IF5A_hypusine_site.
 IPR001884; Transl_elong_IF5A.
 IPR020189; Transl_elong_IF5A_C.
 IPR008991; Translation_prot_SH3-like. 
Pfam
 PF01287; eIF-5a
 PF00467; KOW 
SMART
  
PROSITE
 PS00302; IF5A_HYPUSINE 
PRINTS