CPLM-019130

Genbank Nucleotide ID

Gamma-tubulin complex component 3

Protein Synonyms/Alias

GCP-3; hGCP3; Gamma-ring complex protein 104 kDa; h104p; hGrip104; Spindle pole body protein Spc98 homolog; hSpc98

Homo sapiens (Human)

Position	Peptide	Type	References
220	SSQATTSKGVPSAVS	ubiquitination	[1, 2, 3, 4, 5]
264	VFQGIDGKNIKMNNT	ubiquitination	[1, 3, 4, 5]
267	GIDGKNIKMNNTENC	ubiquitination	[3, 5]
276	NNTENCYKVEGKANL	ubiquitination	[2, 3, 5]
280	NCYKVEGKANLSRSL	ubiquitination	[1, 3]
303	ELGWLHNKIRRYTDQ	ubiquitination	[1, 2, 3, 4, 6, 7, 8]
375	LVWTYDPKIRLKTLA	ubiquitination	[1, 3]
379	YDPKIRLKTLAALVD	ubiquitination	[3, 5]
392	VDHCQGRKGGELASA	ubiquitination	[5]
405	SAVHAYTKTGDPYMR	ubiquitination	[1]
455	VASDPTVKTDRLWHD	ubiquitination	[1]
463	TDRLWHDKYTLRKSM	ubiquitination	[6, 8]
482	MTMDQSRKVLLIGKS	ubiquitination	[3]
505	HDQTPTTKMIAVTKS	ubiquitination	[3]
543	AAYFETSKYLLDVLN	ubiquitination	[1]
551	YLLDVLNKKYSLLDH	ubiquitination	[1, 4, 6, 8]
689	KGHMCNAKLLRNMPE	ubiquitination	[2, 3]
831	AEEEEENKRIGEFKE	ubiquitination	[1]
837	NKRIGEFKESIPKMC	ubiquitination	[1, 3]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005814; C:centriole; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005827; C:polar microtubule; IDA:UniProtKB.
GO:0043015; F:gamma-tubulin binding; IDA:UniProtKB.
GO:0005200; F:structural constituent of cytoskeleton; NAS:UniProtKB.
GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
GO:0007020; P:microtubule nucleation; NAS:UniProtKB.
GO:0007338; P:single fertilization; NAS:UniProtKB.

IPR015697; GCP3.
IPR007259; Spc97_Spc98.

PF04130; Spc97_Spc98